李亚玲,朱克诚,刘宝锁,郭华阳,郭梁,张楠,杨静文,江世贵,张殿昌

• 1:中国水产科学研究院南海水产研究所/农业部南海渔业资源开发利用重点实验室
• 2:中国农业科学院研究生院
• 3:广东省海洋生物种业工程技术研究中心

摘要(Abstract)：

【目的】研究NKCC1a基因在急性盐度胁迫下对黄鳍棘鲷（Acanthopagrus latus）的渗透压调控机理。【方法】用生物信息学软件分析NKCC1a的序列特征，用实时荧光定量PCR(qRT-PCR)技术检测其在黄鳍棘鲷各组织内的表达情况及其在盐度0、8、16、24（对照）和32条件下的表达模式。【结果】NKCC1a开放阅读框（ORF）大小为3 435 bp，共编码1 144个氨基酸，存在一个经典的Na+-K+-Cl-协同转运蛋白SLC12A结构域，且在不同物种中有高保守性。NKCC1a在13个组织中均有表达，在脑中表达量最高，显著高于其他组织（P <0.05）；其次为鳃，在性腺和肝脏中表达量则显著低于其他组织（P<0.05）。盐度胁迫6h时，在淡水组（盐度0）中NKCC1a表达量显著上升（P <0.05）；在盐度为8和16处理组中，NKCC1a表达量先降低后逐渐升高，随后趋于平稳；在盐度32处理组，表达量呈先升高后降低最后又升高趋势，在24 h时最大。【结论】NKCC1a基因在黄鳍棘鲷盐度适应中发挥重要作用。

关键词(KeyWords)： 黄鳍棘鲷;NKCC1a;基因表达;急性盐度胁迫

Abstract:

Keywords:

基金项目(Foundation): 农业部财政重大专项(NHYYSWZZZYKZX2020);; 广东省现代农业产业技术体系海水鱼产业创新项目(2019KJ143)

作者(Author): 李亚玲,朱克诚,刘宝锁,郭华阳,郭梁,张楠,杨静文,江世贵,张殿昌

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