玉洁莹,黄琳,徐伟强,韦云依,王一兵,程淑,蔡佳,刘政昭,陈福彩,李鹏飞

• 1:广西科学院
• 2:广西海洋科学院
• 3:广西水产生物技术与现代生态养殖重点实验室
• 4:广西渔业重大疫病防控与高效健康养殖产业技术工程研究中心

摘要(Abstract)：

【目的】探究五倍子（Galla chinensis）醇提物在石斑鱼（Epinephelus spp.）虹彩病毒（Singapore grouper iridovirus,SGIV）感染中的抗病毒效果及作用机制，为开发抗SGIV药物提供参考。【方法】通过测定细胞活力、观察细胞骨架等方法确定五倍子醇提物对石斑鱼脾脏组织细胞系（Grouper spleen cells,GS）的安全浓度，利用结晶紫染色和实时荧光定量PCR(RT-qPCR)等方法评估五倍子醇提物在细胞水平上对SGIV的抗病毒效果，进一步通过石斑鱼活体试验验证五倍子醇提物的抗SGIV效果。结合RT-qPCR和流式细胞仪的结果，分析五倍子醇提物的抗SGIV机制。【结果】当五倍子醇提物质量浓度≤12.5μg/mL时对GS细胞无显著毒性，GS细胞形态无异常。12.5、6.25、3.125μg/mL的五倍子醇提物均可降低SGIV的感染力，抗病毒效果呈现剂量依赖性。饲料中添加五倍子醇提物可使石斑鱼感染SGIV后肝脏组织内主要衣壳蛋白基因（mcp）的相对表达量显著下降（P <0.01）。五倍子醇提物可与SGIV病毒粒子相互作用，使mcp和囊膜蛋白基因（vp19）的相对表达量显著降低（P <0.01），病毒滴度显著下降（P <0.05）；五倍子醇提物还可干扰SGIV感染细胞过程中的吸附、侵入和复制，药物组细胞中SGIV mcp和vp19基因的相对表达量显著降低（P <0.01）。【结论】五倍子醇提物对SGIV具有良好的体外和体内抗病毒效果，在SGIV侵染宿主细胞的吸附、侵入和复制阶段中起到干扰作用，有抗SGIV感染作用，具有成为安全、高效的抗SGIV新型渔用药物的潜力。

关键词(KeyWords)： 石斑鱼虹彩病毒(SGIV);五倍子;抗病毒机制;渔用药物

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（32373175）;; 广西自然科学基金（2024GXNSFFA010017,2022GXNSFBA035521,2022JJA130074）;; 国家重点研发计划（2022YFD2401200）;; 国家现代农业产业技术体系广西创新团队项目（nycytxgxcxtd-2021-08-02）

作者(Author): 玉洁莹,黄琳,徐伟强,韦云依,王一兵,程淑,蔡佳,刘政昭,陈福彩,李鹏飞

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