一株H5N1亚型禽流感病毒株NA基因的克隆与序列分析Cloning and Sequence Analysis of NA Gene of a H5N1 Subtype Avian Influenza Virus Isolate
陈绍红,刘艳芬,张静,刘铀
摘要(Abstract)：
用RT-PCR方法从1个H5N1亚型禽流感病毒分离株A/Chicken/Guangdong/DH/1997扩增NA基因cDNA片段,将其克隆至pMD18-T载体,获得重组质粒pMD-NA,并对其核苷酸序列进行测定和分析。结果表明,该毒株的NA基因长度为1350bp,编码449个氨基酸,与其它H5N1亚型AIV分离株的核苷酸序列同源性为97.0%～99.4%,氨基酸序列同源性为97.7%～99.1%,提示禽流感病毒NA基因保守性较高。NA基因氨基酸序列的聚类分析表明该毒株与来自香港的A/Pheasant/HK/FY155/01和A/Ch/HK/FY150/01两个分离株处于同一进化枝,亲缘关系较近。
关键词(KeyWords)： 禽流感病毒(AIV);H5N1亚型;NA基因;克隆;序列分析
基金项目(Foundation):
作者(Author): 陈绍红,刘艳芬,张静,刘铀
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