张丽欣,梁旭方,武佳琪

• 1:华中农业大学水产学院/鳜鱼研究中心

摘要(Abstract)：

【目的】分析翘嘴鳜（Siniperca chuatsi）早期基因c-fos的两个亚型fosaa和fosab的分子特征，探究二者学习记忆功能。【方法】根据翘嘴鳜c-fos编码区设计引物，克隆翘嘴鳜fosab基因，通过翘嘴鳜全基因组数据库获取fosaa基因，用生物信息学分析翘嘴鳜fosaa、fosab的结构特征，用荧光定量PCR检测fosaa、fosab在翘嘴鳜健康组织以及投喂饲料7、56 d后脑组织中的表达量。【结果】fosab全长2 498 bp，开放阅读框（ORF）1 152 bp，与fosaa基因相同，有4段外显子；二者基因序列差异较大，DNA序列及氨基酸序列相似性分别为40.4%、46.0%。进化树分析显示，翘嘴鳜FOSAB与其他鱼类以及人（Homo sapiens）、褐家鼠（Rattus norvegic）、小鼠（Mus musculus）和野猪（Sus scrofa）等哺乳动物的C-FOS聚为一支，所有动物FOSAA聚为一支，翘嘴鳜FOSAB蛋白与哺乳类动物C-FOS亲缘关系比与FOSAA的关系更近。同线性分析显示，c-fos基因进化较为保守，处于tmed10和jdp基因之间。荧光定量分析表明，翘嘴鳜fosaa和fosab基因在脑和皮肤中均有较高的表达量，fosaa基因在脑中表达量最高，而fosab基因在皮肤中表达量最高。经饲料驯食后，驯化56 d时fosaa基因表达量显著高于驯化7 d(P <0.05)，而驯化7、56 d的fosab基因相对表达量无显著差异（P> 0.05）。【结论】翘嘴鳜fosaa和fosab基因分子特征差异较大，发挥的功能不尽相同，学习记忆相关基因fosaa在56 d饲料驯食中有重要作用。

关键词(KeyWords)： c-fos;克隆;组织表达;进化分析;学习记忆

Abstract:

Keywords:

基金项目(Foundation): 湖北省重点研发计划（2020BBA053）

作者(Author): 张丽欣,梁旭方,武佳琪

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