潘金露,韩雨哲,霍圃宇,苏鹏,姜志强

• 1:大连海洋大学农业部北方海水增养殖重点实验室

摘要(Abstract)：

在基础饲料中添加质量分数0、0.02%、0.05%、0.20%、0.50%的褐藻酸寡糖,饲喂体质量(17.61±0.16)g的大菱鲆(Scophthalmus maximus L)70 d,研究褐藻酸寡糖对大菱鲆肠道结构、消化酶活性及表观消化率的影响。结果表明:1)褐藻酸寡糖处理组的肠道微绒毛长度与对照组差异无统计学意义(P>0.05),肠道皱襞高度与对照组差异无统计学意义(P>0.05),杯状细胞大于对照组(P<0.05);2)饲料中添加褐藻酸寡糖对大菱鲆肠道内蛋白酶和淀粉酶活性影响无统计学意义(P>0.05),0.02%和0.05%褐藻酸寡糖实验组中的脂肪酶活性与对照组差异有统计学意义(P<0.05);3)褐藻酸寡糖对饲料干物质消化率、蛋白质消化率以及脂肪消化率影响无统计学意义(P>0.05)。在大菱鲆饲料中添加褐藻酸寡糖可增大肠道杯状细胞,促进肠道脂肪酶活性,但对肠绒毛长度,皱襞高度,淀粉酶、蛋白酶活性,表观消化率无影响。

关键词(KeyWords)： 大菱鲆;褐藻酸寡糖;肠道组织切片;消化酶活性;消化率

Abstract:

Keywords:

基金项目(Foundation): 国家海洋局公益性行业科研专项(201405003)

作者(Author): 潘金露,韩雨哲,霍圃宇,苏鹏,姜志强

参考文献(References)：

• [1]刘爱君,冷向军,李小勤,等.甘露寡糖对奥尼罗非鱼(Oreochromis×O.aureus)生长、肠道结构和非特异性免疫的影响[J].浙江大学学报(农业与生命科学版),2009,35(3):329-336.
• [2]王媛媛,郭文斌,宋存江,等.褐藻酸寡糖的生物活性与应用研究进展[J].食品与发酵工业,2010,36(10):122-126.
• [3]董晓莉,耿美玉,管华诗,等.褐藻酸性寡糖对帕金森病大鼠纹状体、杏仁核多巴胺释放的影响[J].中国海洋药物,2003(5):9-12.
• [4]YAN G L,GUO Y M,YUAN J M,et al.Sodium alginate oligosaccharides from brown algae inhibit salmonella enteritidis colonization in broiler chickens[J].Poultry Science,2011,90:1441-1448.
• [5]陈丽,王淑军,刘泉,等.褐藻寡糖对3种水产致病菌抗菌活性研究[J].淮海工学院学报(自然科学版),2009,18(1):90-92.
• [6]江晓路,杜以帅,王鹏,等.褐藻寡糖对刺参体腔液和体壁免疫相关酶活性变化的影响[J].中国海洋大学学报,2009,39(6):1188-1192.
• [7]王鹏,江晓路,江艳华,等.褐藻酸低聚糖对提高大菱鲆免疫机能的作用[J].海洋科学,2006,30(8):6-9.
• [8]郝甜甜,张利民,李宝山,等.饲料中添加糖萜素对大菱鲆幼鱼体成分、消化酶活力、血脂及肠道组织结构的影响[J].上海海洋大学学报,2015,24(3):391-402.
• [9]霍圃宇,潘金露,韩雨哲,等.褐藻酸寡糖对大菱鲆幼鱼生长性能、血液学指标及非特异性免疫影响[J].广东海洋大学学报,2015,35(4):10-16.
• [10]HU H B,ZHANG YJ,MAI K S,et al.Effects of dietary stachyose on growth performance digestive enzyme activities and intestinal morphology of juvenile turbot(Scophthalmus maximus L)[J].Journal of Ocean University of China(Oceanic and Coastal Sea Research),2015,14(5):905-912.
• [11]LOWRY O H,ROSEBROUGH N J,FARR A L,et al.Protein measurement with the folin phenol reagent[J].Biology Chemistry,1951,193:265-275.
• [12]黄锡荣,张文娟,宁少芳,等.分光光度法测定微乳液中脂肪酶的酶活[J].化学通报,2001(10):659-661.
• [13]林浩然.鱼类生理学[M].广州:广东高等教育出版社,2007.
• [14]ZHOU Q C,ALEJANDRO B.Effects of dietary prebiotics on growth performance,immune response and intestinal morphology of red drum(Sciaenops ocellatus)[J].Aquaculture,2010,309:253-257.
• [15]SALZE G,MCLEAN E,SCHWARZ M H,et al.Dietary mannan oligosaccharide enhances salinity tolerance and gut development of larval cobia[J].Aquaculture,2008,274:148-152.
• [16]DIMITROGLOU A,DAVIES S,SWEETMAN J.The effect of dietary mannan oligosaccharides on the intestinal histology of rainbow trout(Oncorhynchus mykiss)[J].Comparative Biochemistry and Physiology,Part A:Molecular&Integrative Physiology,2008,150(3):349-361.
• [17]PRYOR G S,ROYES J B,CHAPMAN F A,et al.Mannanoligosaccharides in fish nutrition:Effects of dietary supplementation on growth and gastrointestinal villi structure in gulf of Mexico sturgeon[J].North American Journal of Aquaculture,2003,65:106-111.
• [18]DIMTROGLOU A,MERRIFIELD D L,MOATE R,et al.Dietary mannan oligosacchaide supplementation modulates intestinal microbial ecology and improves gut morphology of rainbow trout Oncorhynchus mykiss(Walbaum)[J].American society of animal science,2009,87:3226-3234.
• [19]江永明,付天玺,张丽,等.微生物制剂对奥尼罗非鱼生长及消化酶活性的影响[J].水生生物学报,2011,35(6):998-1004.
• [20]杨晴,杨锐,周歧存,等.褐藻糖胶对黄颡鱼幼鱼生长性能和消化酶活性的影响[J].动物营养学报,2014,26(7):1880-1887.
• [21]SANG H M,FOTEDAR R,FILER K.Effects of dietary mannan oligosaccharide on the survival,growth,immunity and digestive enzyme activity of freshwater crayfish,Cherax destructor Clark(1936)[J].Aquaculture Nutrition,2011,17:629-635.
• [22]肖明松,陈庆榆,鲍方印,等.果寡糖对鲤鱼生长性能及消化酶的影响[J].水利渔业,2005,25(6):29-31.
• [23]LIANG R J,SHI S D,ZHANG B J.The effect of fructo-oligosaccharides on blood RBC count and digestive enzyme activities of oxyeleotris lineolatus[J].African Journal of Micobiology Research,2010,4(18):1909-1913.
• [24]MARITZA A,CAMILO P,ALEJANDRO B,et al.The effects of prebiotics on the digestive enzymes and gut histomorphology of red drum(Sciaenops ocellatus)and hybrid striped bass(Morone chrysops×M.saxatilis)[J].British Journal of Nutrition,2013,109:623-629.
• [25]MASLOWSKI K M,MACKAY C R.Diet,gut microbiota and immune responses[J].Nature Immunology,2010,12:5-9.
• [26]明建华,叶金云,张易祥,等.2龄青鱼对7种饲料原料中营养物质的表观消化率[J].动物营养学报,2014,26(1):161-169.
• [27]MANSOURM R,AKRAMI R,GHOBADI S H,et al.Effect of dietary mannan oligosaccharide(MOS)on growth performance,survival,body composition,and some hematological parameters in giant sturgeon juvenile(Huso huso Linnaeus,1754)[J].Fish Physiol Biochem,2012,38:829-835.
• [28]BURR G,HUME M,Neill W H,et al.Effects of prebiotics on nutrient digestibility of a soybean-meal-based diet by red drum Sciaenops ocellatus(Linnaeus)[J].Aquaculture Research,2008,39:1680-1686.
• [29]YE J D,WAND K,LIF D,et al.Single or combined effects of fructo-and mannan oligosaccharide supplements and Bacillus clausii on the growth,feed utilization,body composition,digestive enzyme activity,innate immune response and lipid metabolism of Japanese flounder Paralichthys olivaceus[J].Aquaculture Nutrition,2011,17:902-911.
• [30]蔡立胜,吴天星,潘晓东,等.肉毒碱和果寡糖对鱼幼鱼消化率影响的研究[J].饲料工业,2007,28(4):25-26.
• [31]YILMAZ E,GENC M A,GENC E.Effects of Dietary mannan oligosaccharides on growth,body composition and intestine and liver histology of rainbow trout,Oncorhynchus mykiss[J].The Israeli Journal of Aquaculture-Bamidgeh,2007,59(3):182-188.
• [32]GENC M A,YILMAZ E,GENC E,et al.Effects of dietary mannan oilosacchrides(MOS)on growth,body composition,and intestine and liver histology of the hybrid tilapla(Oreochromis niloticus×O.aureus)[J].The Israeli Journal of Aquaculture-Bamidgeh,2007,59(1):10-16.