黄荣莲,范闪闪,杜晓东

• 1:广东海洋大学水产学院//广东省珍珠养殖与加工工程技术研究中心

摘要(Abstract)：

【目的】研究马氏珠母贝(Pinctada martensii)贝壳基质蛋白(Shell Matrix Proteins, SMPs)基因(Pm-PNU7)表达与功能。【方法】利用马氏珠母贝基因组和蛋白质组信息分析和RACE技术克隆获得壳基质蛋白新基因Pm-PNU7全长序列,通过原位杂交对其进行定位,RNA干扰检测其对贝壳形成的影响。【结果】Pm-PNU7的cDNA全长为797 bp,编码145个氨基酸,具有特殊的"KGG"重复序列和富含天冬氨酸(Asp)序列"DDDDDDHDD"。qRT-PCR分析表明,Pm-PNU7基因在外套膜边缘区和套膜区显著高表达(P<0.05);ISH检测显示,Pm-PNU7主要定位于边缘区外褶外侧和内侧上皮细胞、中褶内侧上皮细胞以及套膜区外侧上皮细胞。RNA干扰后,Pm-PNU7在外套膜边缘区和套膜区的表达量均显著下调,贝壳棱柱层和珍珠层微观结构均出现不同程度的紊乱。【结论】Pm-PNU7参与了贝壳棱柱层和珍珠层的形成。

关键词(KeyWords)： 马氏珠母贝;贝壳基质蛋白;原位杂交;RNA干扰

Abstract:

Keywords:

基金项目(Foundation): 广东省科学技术厅(2017A030303076,2017A030307024);; 国基自然科学基金(31672626)

作者(Author): 黄荣莲,范闪闪,杜晓东

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