夏心怡,彭瑞冰,张聪颖,蒋霞敏

• 1:宁波大学海洋学院藻类生理生化实验室

摘要(Abstract)：

【目的】研究不同光质对新月菱形藻（Nitzschia closterium）生长、总脂含量和脂肪酸组成的影响，确定新月菱形藻生长及油脂产量的最适光质。【方法】在智能光质培养箱的静置培养条件下，采用单因子试验分别研究不同光质（LED白光、LED红光、LED蓝光、LED绿光和LED黄光）条件下新月菱形藻的生长、总脂含量和脂肪酸组成。【结果】光质对新月菱形藻的生长、总脂累积和脂肪酸合成有显著影响（P <0.05）:LED绿光和黄光培养下新月菱形藻比生长速率显著高于白光、蓝光和红光组；LED白光、绿光和黄光培养下新月菱形藻的叶绿素a含量显著高于红光和蓝光组；LED白光、绿光和黄光培养下新月菱形藻的总脂含量显著高于红光和蓝光组；LED蓝光、绿光和黄光培养下新月菱形藻的多不饱和脂肪酸（PUFAs）含量没有显著差异（P> 0.05），显著高于白光和红光组；LED蓝光培养下新月菱形藻的二十碳五烯酸（EPA）含量最高，显著高于其他组；LED红光培养下新月菱形藻的二十二碳六烯酸（DHA）含量最高，显著高于其他组。【结论】LED绿光和黄光能提高新月菱形藻的生长速率，LED蓝光有利于提高新月菱形藻的PUFAs和EPA的含量，LED红光有利于提高新月菱形藻的DHA含量。

关键词(KeyWords)： 新月菱形藻;光质;生长;总脂含量;脂肪酸

Abstract:

Keywords:

基金项目(Foundation): 宁波市自然基金“浙江沿海优质饵料微藻种质资源收集、筛选及高效培养技术研究”（202302N3023）

作者(Author): 夏心怡,彭瑞冰,张聪颖,蒋霞敏

参考文献(References)：

• [1] ZHOU W G, LU Q A, HAN P, et al. Microalgae cultivation and photobioreactor design[M]//Microalgae Cultivation for Biofuels Production. Amsterdam:Elsevier, 2020:31-50.
• [2] TURCIHAN G, TURGAY E, YARD?MC?R E, et al. The effect of feeding with different microalgae on survival,growth, and fatty acid composition of Artemia franciscana metanauplii and on predominant bacterial species of the rearing water[J]. Aquaculture International, 2021, 29(5):2223-2241.
• [3]彭瑞冰,蒋霞敏,乐可鑫,等. 5种饵料动物的营养成分分析及评价[J].水产学报, 2014, 38(2):257-264.
• [4] LI D J, YUAN Y Z, CHENG D J, et al. Effect of light quality on growth rate, carbohydrate accumulation, fatty acid profile and lutein biosynthesis of Chlorella sp. AE10[J]. Bioresource Technology, 2019,291:121783.
• [5]王祎哲,姚奕炯,王梓懿,等.光照对纤细裸藻生长及光合色素含量影响的研究[J].水产科学, 2021, 40(2):179-187.
• [6]陈伟,余杰玲,陈晨远. LED光质组合对衣藻生长的影响[J].科学技术创新, 2020(10):31-32.
• [7]夏建荣,黄瑾.氮、磷对小新月菱形藻无机碳利用与碳酸酐酶活性的影响[J].生态学报, 2010, 30(15):4085-4092.
• [8]薛瑞萍,蒋霞敏,韩庆喜,等.温、光、盐对硅藻STR01生长、总脂、脂肪酸的影响[J].水生生物学报, 2019, 43(3):670-679.
• [9]李鹏飞,孙昕,杨娌,等.藻类叶绿素a提取的优化研究[J].化工学报, 2019, 70(9):3421-3429.
• [10]王盛林,赵震宇,刘平怀. 10种热带富油微藻生物量、总脂含量及脂肪酸组成分析[J].中国油脂, 2017, 42(8):93-97.
• [11] CHEN Q, JIANG X M, HAN Q X, et al. Growth, calcium content, proximate composition, and fatty acid composition of triangle sail mussel(Hyriopsis cumingii)fed five different microalgal diets[J]. Aquaculture, 2021, 530:735719.
• [12]钟彩荣,陈钦常,许涵玥等.光质对等鞭金藻的生长和生物活性产物合成的影响[J/OL].天然产物研究与开发:1-10[2023-08-21]. http://kns. cnki. net/kcms/detail/51.1335.Q.20230726.0831.002.html.
• [13] LI Y F, LI R Q, YI X Y. Effects of light quality on growth rates and pigments of Chaetoceros gracilis(Bacillariophyceae)[J].Journal of Oceanology and Limnology, 2020, 38(3):795-801.
• [14]赵松浩,陶秋爽,沈建仁,等.硅藻岩藻黄素-叶绿素a/c蛋白:揭秘红系捕光天线复合物[J].自然杂志, 2021, 43(3):157-164.
• [15]刁进进.寇氏隐甲藻实验室适应性进化和遗传转化体系建立的研究[D].天津:天津大学, 2018.
• [16]刘春香,李文胜,管宇航,等.高CO2和Cd2+对铜绿微囊藻叶绿素含量及荧光特性影响[J].广东化工, 2021, 48(9):29-31.
• [17]侯红焰,向威鹏,张金荣,等.高产岩藻黄素的海洋硅藻筛选及光照条件优化[J].水生生物学报, 2020, 44(4):912-919.
• [18] KOCHETOVA G V, AVERCHEVA O V, BASSARSKAYA E M, et al. Light quality as a driver of photosynthetic apparatus development[J]. Biophysical Reviews, 2022, 14(4):779-803.
• [19]徐润洁,龚一富,陈文婷,等.不同发光二极管单色光质对三角褐指藻中岩藻黄素含量及相关基因表达的影响[J].光学学报, 2019, 39(9):299-307.
• [20] ATTA M, IDRIS A, BUKHARI A, et al. Intensity of blue LED light:a potential stimulus for biomass and lipid content in fresh water microalgae Chlorella vulgaris[J]. Bioresource Technology, 2013, 148:373-378.
• [21] HULTBERG M, J?NSSON H L, BERGSTRAND K J, et al.Impact of light quality on biomass production and fatty acid content in the microalga Chlorella vulgaris[J]. Bioresource Technology, 2014, 159:465-467.
• [22]柯城,吴春法,李伟军,等.理性认识蓝光危害及蓝光生物安全性评价[J].激光生物学报, 2023, 32(2):97-110.
• [23]许晓路,张德勇.海洋微藻多不饱和脂肪酸生物合成机制的研究进展[J/OL].中国粮油学报:1-11[2023-06-27].https://doi.org/10.20048/j.cnki.issn.1003-0174.000143.
• [24] AFITLHILE M, DUFFIELD-DUNCAN K, FRY M, et al.The toc132toc120 heterozygote mutant of Arabidopsis thaliana accumulates reduced levels of hexadecatrienoic acid[J]. Plant Physiology and Biochemistry, 2015, 96:426-435.
• [25] XIA Y, ZHANG Y T, SUN J Y, et al. Strategies for enhancing eicosapentaenoic acid production:from fermentation to metabolic engineering[J]. Algal Research-Biomass Biofuels and Bioproducts, 2020, 51:102038.
• [26] WANG X J, ZHANG P, WU Y B, et al. Effect of light quality on growth, ultrastructure, pigments, and membrane lipids of Pyropia haitanensis[J]. Journal of Applied Phycology,2020, 32:4189-4197.
• [27] LUPETTE J, JAUSSAUD A, SEDDIKI K, et al. The architecture of lipid droplets in the diatom Phaeodactylum tricornutum[J]. Algal Research, 2019, 38. DOI:10.1016/j.algal.2019.101415.