章双,黎铭,董晓慧,杨奇慧,迟淑艳,刘泓宇,谭北平

• 1:广东海洋大学水产学院
• 2:南海生物资源开发与利用协同创新中心
• 3:广西水产科学研究院广西水产遗传育种与健康养殖重点实验室

摘要(Abstract)：

输入蛋白α(importinα)是核转运蛋白家族的重要成员,可通过帮助具有核定位信号(Nuclear location signal,NLS)蛋白入核而参与免疫过程。克隆并鉴定凡纳滨对虾(Litopenaeus vannamei)的importinα1基因,命名为Lv IMα1,基因全长c DNA为2 181 bp,包括1 575 bp的开放阅读框,编码525个氨基酸,5′非编码区长110 bp,3′非编码区长496 bp。Smart分析显示,Lv IMα1蛋白包含一个N端的输入蛋白β结合域和一个包含8个串联重复序列的NLS中央结合结构域。同源性分析发现,Lv IMα1与原鸡(Gallus gallus)IMα1的相似度最高,为73%;与水蚤(Daphnia magna)IMα1相似度最低,为61%。系统进化分析显示,Lv IMα1和多种无脊椎动物IMα1聚为一类。实时荧光定量PCR(QRT-PCR)分析表明,Lv IMα1在所测试组织中均有表达,在神经组织中表达量最高。白斑综合征病毒感染后,凡纳滨对虾血液中Lv IMα1基因的表达量呈先下降后上升趋势,除感染后4 h外,其余时间点的表达量均低于对照组(P<0.05),12 h最低,为对照组的0.22倍,随后逐渐升高。副溶血弧菌(V.parahaemolyticus)感染后,凡纳滨对虾血细胞中Lv IMα1基因表达量低于对照组,在感染后4、24、48、72 h尤为显著,24 h时最低,为对照组的0.38倍。Lv IMα1基因可能参与凡纳滨对虾抗病免疫应答途径。

关键词(KeyWords)： 凡纳滨对虾;输入蛋白α1;基因;克隆;表达

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金-博士启动项目(2014A030310184);广东省自然科学基金-自由申请项目(2016);; 广东普通高校青年创新人才项目(2014KQNCX079);; 广东省高校珠江学者计划项目;; 广东海洋大学优秀青年骨干教师特别资助计划项目(HDYQ2015004);广东海洋大学自然科学基金

作者(Author): 章双,黎铭,董晓慧,杨奇慧,迟淑艳,刘泓宇,谭北平

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