羟乙基壳聚糖基Pickering乳液制备及静电纺丝工艺优化Preparation and Electrospinning of Hydroxyethyl Chitosan-based Pickering Emulsion
侯婷婷,杨倩,李程鹏,邱洪茂,罗晓燕
摘要(Abstract):
【目的】探究ZnO/Ag复合粒子(ZA)对基于羟乙基壳聚糖(HCS)的Pickering乳液的稳定效果,并通过静电纺Pickering乳液工艺优化制备包埋有茶树精油(TTO)的纳米纤维。【方法】以合成的ZA为稳定剂,研究ZA和TTO用量对HCS乳液稳定性的影响规律;以电压、聚乙烯醇(PVA)用量和接收距离为考察指标,优化纳米纤维加工工艺;以水蒸气透过法和滤纸片法研究所得纳米纤维的透气性能和抗菌活性。【结果】采用质量分数1.5%的ZA和体积分数60%的TTO,可获得最稳定的HCS乳液。最优的成丝条件为电压22kV、接收距离15cm、PVA质量分数10%,此条件下所得纤维的直径约为0.60μm,其对大肠杆菌和金葡菌的抑菌宽度分别为(16.97±0.09)和(15.97±0.11)mm,所得纤维膜的水蒸气透过率为0.21 g/(cm2·d)。【结论】通过ZA的稳定作用,可获得包埋TTO的HCS纳米纤维。
关键词(KeyWords): Pickering乳液;纳米纤维;抗菌活性;茶树油;纳米氧化锌
基金项目(Foundation): 广东省自然科学基金(2020A1515011011);; 广东海洋大学优秀学位论文培育项目(576120035)
作者(Author): 侯婷婷,杨倩,李程鹏,邱洪茂,罗晓燕
参考文献(References):
- [1]HSIEH H T,CHANG H M,LIN W J,et al.Poly-methyl methacrylate/polyvinyl alcohol copolymer agents applied on diabetic wound dressing[J].Scientific Reports,2017,7(1):9531.
- [2]HUAN S Q,LIU G X,HAN G P,et al.Effect of experimental parameters on morphological,mechanical and hydrophobic properties of electrospun polystyrene fibers[J].Materials,2015,8(5):2718-2734.
- [3]YANG J J,HUANG X P,LUO F H,et al.Preparation and functional studies of hydroxyethyl chitosan nanoparticles loaded with anti-human death receptor 5 single-chain antibody[J].Onco Targets and Therapy,2014,7:779-787.
- [4]WANG Y P,QIAN J M,ZHAO N,et al.Novel hydroxyethyl chitosan/cellulose scaffolds with bubble-like porous structure for bone tissue engineering[J].Carbohydrate Polymers,2017,167:44-51.
- [5]LOW W L,KENWARD K,BRITLAND S T,et al.Essential oils and metal ions as alternative antimicrobial agents:a focus on tea tree oil and silver[J].International Wound Journal,2017,14(2):369-384.
- [6]BAZILEVSKY A V,YARIN A L,MEGARIDIS C M.Co-electrospinning of Core-Shell fibers using a single-nozzle technique[J].Langmuir,2007,23(5):2311-2314.
- [7]CHEVALIER Y,BOLZINGER M A.Emulsions stabilized with solid nanoparticles:Pickering emulsions[J].Colloids and Surfaces A:Physicochemical and Engineering Aspects,2013,439:23-34.
- [8]MORSE A J,DUPIN D,THOMPSON K L,et al.Novel Pickering Emulsifiers based on p H-Responsive Poly(tert-butylaminoethyl methacrylate) Latexes[J].Langmuir,2012,28(32):11733-11744.
- [9]HE K H,LI Q,LI Y,et al.Water-insoluble dietary fibers from bamboo shoot used as plant food particles for the stabilization of O/W Pickering emulsion[J].Food Chemistry,2020,310:125925.
- [10]CAI N,HAN C,LUO X G,et al.Fabrication of core/shell nanofibers with desirable mechanical and antibacterial properties by Pickering emulsion electrospinning[J].Macromolecular Materials and Engineering,2017,302(3):1600364.
- [11]NE?OVI?K,JANKOVI?A,KOJI?V,et al.Silver/poly(vinyl alcohol)/chitosan/graphene hydrogelsSynthesis,biological and physicochemical properties and silver release kinetics[J].Composites Part B:Engineering,2018,154:175-185.
- [12]崔巍巍,刘娅,王宗良,等.纳米银/二甲基砜/聚乳酸-乙醇酸静电纺丝人工敷料的制备及生物评价[J].高等学校化学学报,2013,34(3):679-685.
- [13]WU C J,ZHANG G X,XIA T,et al.Bioinspired synthesis of polydopamine/Ag nanocomposite particles with antibacterial activities[J].Materials Science and Engineering:C,2015,55:155-165.
- [14]LIANG L M,HOU T T,OUYANG Q Q,et al.Antimicrobial sodium alginate dressing immobilized with polydopamine-silver composite nanospheres[J].Composites Part B:Engineering,2020,188:107877.
- [15]HOMEYER D C,SANCHEZ C J,MENDE K,et al.In Vitro activity of Melaleuca alternifolia (tea tree) oil on filamentous fungi and toxicity to human cells[J].Medical Mycology,2015,53(3):285-294.
- [16]NICHOLLS S G,OAKLEY C L,TAN A,et al.Demodex treatment in external ocular disease:the outcomes of a Tasmanian case series[J].International Ophthalmology,2016,36(5):691-696.
- [17]胡忆雪,姚雷,黄健,等.茶树油对3种痤疮致病菌的抑制作用研究[J].上海交通大学学报(农业科学版),2011,29(1):88-92.
- [18]IVANOVA T,HARIZANOVA A,KOUTZAROVA T,et al.Preparation and characterization of Zn O-Ti O2 films obtained by Sol-gel method[J].Journal of Non-Crystalline Solids,2011,357(15):2840-2845.
- [19]DREYER D R,MILLER D J,FREEMAN B D,et al.Elucidating the structure of poly(dopamine)[J].Langmuir,2012,28(15):6428-6435.
- [20]FENG J J,ZHANG P P,WANG A J,et al.One-step synthesis of monodisperse polydopamine-coated silver core-shell nanostructures for enhanced photocatalysis[J].New Journal of Chemistry,2012,36(1):148-154.
- [21]CHEN J H,CHENG C Y,CHIU W Y,et al.Synthesis of Zn O/polystyrene composites particles by Pickering emulsion polymerization[J].European Polymer Journal,2008,44(10):3271-3279.
- [22]王莎,贺拥军,李冬.碳酸钙微粒稳定的橄榄油/水乳液及其缓释性能[J].化学研究与应用,2010,22(2):218-221.
- [23]赵强忠,周海媚.大豆纤维稳定水包油型皮克林乳液的研究[J].现代食品科技,2016,32(10):39-44.
- [24]ZOU Y,GUO J,YIN S W,et al.Pickering emulsion gels prepared by hydrogen-bonded zein/tannic acid complex colloidal particles[J].Journal of Agricultural and Food Chemistry,2015,63(33):7405-7414.
- [25]FENG X,DAI H J,MA L,et al.Properties of Pickering emulsion stabilized by food-grade gelatin nanoparticles:influence of the nanoparticles concentration[J].Colloids and Surfaces B:Biointerfaces,2020,196:111294.
- [26]RASOULI M,PIRSALAMI S,ZEBARJAD S M.Study on the formation and structural evolution of bead-on-string in electrospun polysulfone mats[J].Polymer International,2020,69(9):822-832.
- [27]HUAN S Q,LIU G X,HAN G P,et al.Effect of experimental parameters on morphological,mechanical and hydrophobic properties of electrospun polystyrene fibers[J].Materials,2015,8(5):2718-2734.
- [28]陈敏杰.三种植物精油微胶囊的制备及其性能研究[D].广州:华南农业大学,2016.
- [29]SWEENEY I R,MIRAFTAB M,COLLYER G.Acritical review of modern and emerging absorbent dressings used to treat exuding wounds[J].International Wound Journal,2012,9(6):601-612.
- [30]NOROUZI M A,MONTAZER M,HARIFI T,et al.Flower buds like PVA/Zn O composite nanofibers assembly:Antibacterial,in vivo wound healing,cytotoxicity and histological studies[J].Polymer Testing,2021,93:106914.
- [31]张品.茶树油粘胶纤维的制备及其性能测试研究[D].郑州:中原工学院,2014.