胡子鲇3个养殖群体遗传结构的AFLP分析Analysis on Genetic Structure of Three Cultured Populations Claris fuscu with AFLP Markers
邓思平,张艳艳,朱春华,吴天利,师尚丽,张中国,赵志勇,李广丽
摘要(Abstract)：
应用AFLP方法,从64对引物中筛选出5对用于分析胡子鲇(Claris fuscus)厦门(XM)、江门(JM)、玉林(YL)等3个养殖群体共90个个体群体内、群体间遗传多样性。扩增共得到289条清晰谱带,引物多态性介于47.8%～63.8%,平均多态性比率为56.4%。胡子鲇群体总遗传杂合度(Ht)、遗传分化系数(Hs)、遗传分化度(Gst)和基因流(Nm)分别为0.161 1、0.153 6、0.051 1和8.501 8,表明胡子鲇多态性处于中等偏下水平,群体内部遗传分化系数较高,三个群体间无较大分化,群体间基因流动较好。其中,JM群体多态性比率、有效等位基因数Ne、Nei’s指数和Shannon指数都较XM群体和YL群体高,种内分化程度较好。对群体进行两两比较发现,XM群体和YL群体间分化程度最大,XM群体和JM群体次之,JM群体和YL群体最小。基于杂交优势,推断XM和YL群体繁殖所产生的后代将具有比亲代更为优良的性状。
关键词(KeyWords)： 胡子鲇;群体结构;遗传多样性;AFLP
基金项目(Foundation): 广西科学研究与技术开发计划重点项目(桂科合1140009-4)
作者(Author): 邓思平,张艳艳,朱春华,吴天利,师尚丽,张中国,赵志勇,李广丽
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