闫秀英,邢明清,郑风荣,孙修勤,简纪常,吴灶和

• 1:广东海洋大学广东省水产经济动物病原生物学及流行病学重点实验室
• 2:青岛市市立医院
• 3:国家海洋局第一海洋研究所
• 4:仲恺农业工程学院

摘要(Abstract)：

海水养殖生物病毒病是世界性难题,淋巴囊肿病毒中国分离株(LCDV-cn)是牙鲆(Paralichthys olivaceus)淋巴囊肿病的病原。以mcp基因为靶基因,设计siRNA-mcp1、siRNA-mcp2、siRNA-mcp3等3条siRNA序列,研究RNA干扰(RNAi)对淋巴囊肿病毒在牙鲆体内复制的影响。结果表明,牙鲆体内注入siRNA-mcp1和siRNA-mcp3后,mcp基因的表达有所下调;统计分析表明,注入siRNA-mcp1后,mcp基因的表达下调明显,具有显著性差异(p<0.05),注入siRNA-mcp2、siRNA-mcp3后,mcp基因的表达下调不明显,差异不显著(p>0.05)。本实验中初步实现了RNAi对LCDV-cn复制的抑制作用。

关键词(KeyWords)： 淋巴囊肿病毒中国分离株(LCDV-cn);mcp基因;RNA干扰

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划项目(2006AA100309)

作者(Author): 闫秀英,邢明清,郑风荣,孙修勤,简纪常,吴灶和

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