范嗣刚,刘勇,赵超,侯思禹,邱丽华

• 1:中国水产科学研究院南海水产研究所/广东省渔业生态环境重点实验室/农业农村部水产品加工重点实验室
• 2:上海海洋大学水产与生命学院
• 3:天津农学院水产学院

摘要(Abstract)：

【目的】了解核因子κB抑制蛋白激酶ε2(IKKε2)基因在日本囊对虾（Marsupenaeusjaponicus）受细菌刺激后的应答情况，以阐释IKKε2在对虾先天免疫中所起的作用。【方法】从日本囊对虾转录组数据中获得日本囊对虾IKKε2（命名为PjIKKε2）的cDNA序列，并进行生物信息学分析，采用荧光定量PCR分析PjIKKε2的组织表达以及受哈维弧菌（Vibrio harveyi）刺激后在肝胰腺组织的表达情况。【结果】PjIKKε2全长为4 009 bp，其中开放阅读框长2 337 bp，编码778个氨基酸，5′UTR(Untranslated region)174 bp,3′UTR 1 498 bp。PjIKKε蛋白含有一个激酶结构域、一个类泛素结构域和一个TANK结合激酶卷曲螺旋结构域。多重序列比对结果显示，PjIKKε2蛋白序列与其他甲壳类IKKε的相似性高，在激酶结构域保守性高。系统进化树结果显示，PjIKKε2与其他对虾属的IKKε聚集在一支。PjIKKε2在日本囊对虾9个组织中均有表达，在淋巴组织中表达量最高（P <0.05）。在哈维弧菌刺激6 h后，PjIKKε2在肝胰腺中表达量显著上调，达到最大值（P <0.05）。【结论】PjIKKε2参与了日本囊对虾的先天免疫。

关键词(KeyWords)： 日本囊对虾;核因子κB抑制蛋白激酶ε2;基因表达;先天免疫

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金（31802281）;; 广东省重点领域研发计划项目（2021B0202020002）;; 中国水产科学研究院基本科研业务费专项(2020TD21);; 广州市科技计划项目（202102020487）;; 广州市农村科技特派员项目（20212100051）;; 中央级公益性科研院所基本科研业务费专项(2021TS03;2021SD11)

作者(Author): 范嗣刚,刘勇,赵超,侯思禹,邱丽华

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