何飞祥,江东能,陈华谱,邓思平,吴天利,田昌绪,石红娟,朱春华,李广丽

• 1:广东海洋大学水产学院//南海水产经济动物增养殖广东省普通高校重点实验室//广东省名特优鱼类生殖调控与繁育工程技术研究中心//湛江市海洋生态与养殖环境重点实验室

摘要(Abstract)：

【目的】分析金钱鱼(Scatophagus argus)卵巢发育过程重要基因——合子阻滞因子1(Zygote arrest 1,Zar1)组织表达及其在卵子成熟和胚胎发育过程中的表达。【方法】克隆金钱鱼Zar1基因,采用反转录-PCR(RT-PCR)检测其在各组织的分布情况,实时荧光定量PCR(q PCR)研究Zar1在不同卵巢发育时期和胚胎发育过程中的表达。【结果】金钱鱼Zar1的开放阅读框(ORF)序列全长为1008bp,编码335个氨基酸。序列分析发现,金钱鱼Zar1在蛋白C末端高度保守,具有非典型的PHD基序(Plant homeo domain)和锌指结构域。金钱鱼Zar1同源性分析发现,它与鲈形目物种相似度最高,其中大黄鱼(Larimichthys crocea)相似性最高,为85.1%。系统进化树分析显示,金钱鱼Zar1与大黄鱼亲缘关系最近,与其分类地位一致。组织分布发现,金钱鱼Zar1仅在卵巢中表达。Zar1在II、III和IV期卵巢表达呈现逐渐递增趋势,其中IV期表达水平显著高于II期卵巢。Zar1在胚胎发育早期表达较高,后期较低,其中在四细胞期表达水平最高,之后逐渐降低。【结论】金钱鱼Zar1在卵巢和胚胎期表达,在金钱鱼卵子发生和胚胎发育阶段发挥重要作用。

关键词(KeyWords)： 金钱鱼;Zar1;克隆;卵子发生;胚胎发育

Abstract:

Keywords:

基金项目(Foundation): 湛江市财政资金科技竞争性分配项目(2016A03017);; 广东省自然科学基金(2016A030313743)

作者(Author): 何飞祥,江东能,陈华谱,邓思平,吴天利,田昌绪,石红娟,朱春华,李广丽

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