吴羽媛,郭志颖,梁海鹰,王庆恒,邓岳文,杜晓东

• 1:广东海洋大学水产学院
• 2:广东省珍珠养殖与加工工程技术研究中心

摘要(Abstract)：

Lec-8(C-type lectin 8)属于C-型凝集素超家族,在机体抵抗微生物的感染过程起重要的作用。本研究采用RACE技术克隆出了PmLec-8基因cDNA全长序列,并且运用实时荧光定量PCR(qRT-PCR)技术检测了PmLec-8基因在马氏珠母贝(Pinctada fucata martensii)不同组织中的表达模式。结果显示:PmLec-8基因序列全长737 bp,其中5′UTR长为36 bp,3′UTR长为113 bp,开放阅读框(ORF)为588 bp,编码195个氨基酸,预测其分子质量约为22.90 ku,等电点为5.17;PmLec-8具在一个糖类识别结构域(Carbonhydrate-recognition domain,CRD),符合典型的C型凝集素(C-type lectin)家族特征;多序列比对结果表明物种间Lec-8的同源性较低,4个半胱氨基酸残基高度保守;qRT-PCR数据分析表明,PmLec-8在马氏珠母贝肝胰腺、性腺、闭壳肌、鳃、血细胞和外套膜中均有表达,其中肝胰腺中表达量最高,其次是鳃,差异具统计学意义(P<0.05)。

关键词(KeyWords)： 马氏珠母贝;C-型凝集素;基因克隆;实时荧光定量PCR

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31472306);; 广东省科技计划项目(2012A031100010);; 广东省海港建设与渔业产业发展专项(A201608B15);; 国家级大学生创新创业训练项目(201410566005);; 广东海洋大学大学生创新创业训练项目(CXXL2014012)

作者(Author): 吴羽媛,郭志颖,梁海鹰,王庆恒,邓岳文,杜晓东

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