李亚

• 1:广东海洋大学农学院

摘要(Abstract)：

通过绿僵菌属甘露糖6-磷酸异构酶基因保守核苷酸区域设计简并性引物,采用RT-PCR及RACE-PCR技术成功克隆了金龟子绿僵菌mpi基因cDNA序列。该基因cDNA序列全长为1 513 bp,开放阅读框长度为1 328 bp,共编码441氨基酸。BLAST分析发现该基因演绎的氨基酸序列与其它真菌同源性较高,蛋白结构分析表明MPI蛋白是较保守的蛋白磷酸酶结构特征,主要由α螺旋和不规则卷曲构成。

关键词(KeyWords)： 金龟子绿僵菌;RACE;甘露糖6-磷酸异构酶基因;克隆

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金(9452408801002439);; 湛江市科技计划项目

作者(Author): 李亚

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