刘丽;陈育盛;李晓娜;申玉春;刘楚吾;

• 1:广东海洋大学水产学院南海水产经济动物增养殖广东省普通高校重点实验室

摘要(Abstract)：

通过PCR扩增直接测序得到10种徐闻石珊瑚线粒体Cyt b基因部分序列,分析其碱基组成和变异频率,并采用Neighbor-Joining和Maximum Parsimony法构建系统发育树。结果显示:序列中A+T比例为61.0%,G+C比例为39.0%,前者明显高于后者,碱基替换主要发生在密码子第3位;滨珊瑚科分类与传统分类存在一定差异,揭示传统形态学分类可能受珊瑚骨骼生长可塑性限制,造成分类不准确;刺柄珊瑚属与蜂巢珊瑚科各属亲缘关系较近。

关键词(KeyWords)： 石珊瑚;Cytb基因;碱基比例;变异频率;系统发育树

Abstract:

Keywords:

基金项目(Foundation): 国家海洋公益性行业科研专项(201105012);; 广东省海洋渔业科技推广专项(A200908F01);; 广东省自然科学基金(8452408801001272)

作者(Authors): 刘丽;陈育盛;李晓娜;申玉春;刘楚吾;

参考文献(References)：

• [1]Romano S L,Stake J L.The phylum Cnidaria:A review of phylogenetic patterns and diversity 300 years after Linnaeus[J].Zootaxa,2007,1668:127-182.
• [2]Fukami H.,Budd A.F.,Paulay G.,et al.Conventional taxonomy obscures deep divergence between Pacific and Atlantic corals[J].Nature,2004,427(6977):832-835.
• [3]Forsman Z H,Concepcion G T,Haverkort R D,et al.Genetic and morphological characterization of a coral Species of Concern,Montipora dilatata,in Kaneohe Bay,Oahu,Hawaii[R].Hawaii Institute of Marine Biology:Pacific Islands Region Species of Concern Project Report,2008:1-17.
• [4]张秀梅,柳广东,高天翔,等.绒鳌蟹种质资源研究进展[J].青岛海洋大学学报,2002,32(4):533-542.
• [5]郑聪霞,姜乃澄,沈琴芳.海葵目4种海葵线粒体cyt b基因的序列分析[J].浙江大学学报,2010,37(1):87-91.
• [6]朱世华,杨迎春,郑文娟,等.从细胞色素b部分序列探讨石斑鱼属的分子系统发育关系[J].水生生物学报,2006,30(4):432-438.
• [7]Ma Guo-long,Chang Hong,Li Shi-ping,et al.Phylogenetic relationships and status quo of colonies for gayal based on analysis of cytochrome b gene partial sequences[J].Journal of Genetics and Genomics,2007,34(5):413-419.
• [8]Van Oppen M J H,Willis B L,Miller D J.Atypically low rate of cytochrome b evolution in the scleractinian coral genus Acropora[J].Proc.R.Soc.Lond.B,1999,266(1):179-183.
• [9]朱弘复,郑作新,黄大卫,等.中国动物志?造礁石珊瑚[M].北京科学出版社,1997:127-228.
• [10]卢圣栋.现代分子生物学实验技术[M].北京:中国协和医科大学出版社,1999:61.
• [11]Rubin E T,Moulding A L,Lopez J V,et al.Scleractinian coral recruitment to reefs physically damaged by ship groundings[C]//.Proceedings of the 11th International Coral Reef Symposium,Ft.Lauderdale,Florida,July 7-11,2008(Session number 10).2008,1:326-330.
• [12]Berntson E A,Bayer F M,Mcarthur A G,et al.Phylogenetic relationships within the octocorallia(cnidaria:anthozoa)based on nuclear 18S rDNA sequences[J].Mrine biology,2001,138(2):235-246.
• [13]Sinniger F,ChevaldonnéP,Pawlowski J..Mitochondrial genome of Savalia savaglia(Cnidaria,Hexacorallia)and early metazoan phylogeny[J].Journal of Molecular Evolution,2007,64(2):196-203.
• [14]Zardoya R,Meyer A.Phylogenetic performance mitochondrial protein coding genes in resolving relationship among vertebrate[J].Molecular Biology and Evolution,1996,13(7):933-942.
• [15]张大治,杨贵军,郑哲民.基于线粒体细胞色素b基因序列的蜻亚科部分种类分子系统学研究[J].宁夏大学学报,2006,27(3):254-259.
• [16]Crozier R H,Crozier Y C.The cytochrome b and ATPase genes of honeybee mitochondria DNA[J].Mol.Biol.Evol.,1992,9(3):474-482.
• [17]Hugall.Evolution of the AT-rich mitochondrial DNA of the root knot nematode Meloidogyne hapia[J].Mol.Biol.Evol.,1997,14(1):40-48.
• [18]Peter A,Todd.Morphological plasticity in scleractinian corals[J].Biological Reviews,2008,83(3):315-337.
• [19]Forsman Z H,Barshis D J,Hunter C L,et al.Shape-shifting corals:Molecular markers show morphology is evolutionarily plastic in Porites[J].BMC Evolutionary Biology,2009,9:45.
• [20]Romano S L,Palumbi S R.Molecular evolution of a portion of the mitochondrial 16S ribosomal gene region in scleractinian corals[J].J Mol Evol,1997,45(4):397-411.
• [21]戴昌凤,洪圣雯.台湾珊瑚图鉴[M].台北:猫头鹰出版社,2009:153.