胡娜,何苹萍,张兴志,官俊良,韦嫔媛,张立,李蔚,郑玉斯,白科,陈泳先,李文红,彭金霞

• 1:广西大学动物科学技术学院
• 2:广西壮族自治区水产科学研究院/农业农村部中国(广西)-东盟水产种质资源综合开发与利用重点实验室/广西水产遗传育种与健康养殖重点实验室

摘要(Abstract)：

【目的】挖掘与香港牡蛎（Crassostrea hongkongensis）生长相关的候选基因和相关通路，为深入探索香港牡蛎的生长调控机制提供基础数据。【方法】分别选取3只快速生长组[FG,(173.23±12.85)g]和3只慢速生长组[SG,(123.87±10.98)g]的闭壳肌组织进行转录组测序。使用DESeq2分析样品组间的基因差异表达，对差异基因进行Kmeans聚类分析，KEGG和GO功能富集性分析。【结果与结论】测序共获得354 605 398个原始测序序列（Raw reads），经过滤和筛选，得到340 597 178个高质量数据（Clean reads）。根据|log_2差异倍数|≥1和错误发现率（FDR）<0.05的筛选标准，共鉴定出34个差异表达基因，其中24个表达上调基因，10个表达下调基因。结合基因功能注释信息分析，共筛选出ARA、CO6A4、DTX3、KY、MKX、BIN3、TRI33和5HT4R等8个与生长相关的候选基因。GO功能富集分析显示，注释差异基因数目较多的有细胞解剖实体、结合和细胞过程等条目。此外，差异基因也显著富集在Notch信号通路、ECM-受体相互作用以及神经活性配体-受体相互作用等3条重要的KEGG通路。

关键词(KeyWords)： 香港牡蛎;转录组测序;差异表达基因;生长

Abstract:

Keywords:

基金项目(Foundation): 国家现代农业产业技术体系广西创新团队项目（nycytxgxcxtd-2023-14）;; 国家贝类产业技术体系钦州综合试验站项目（CARS-49）

作者(Author): 胡娜,何苹萍,张兴志,官俊良,韦嫔媛,张立,李蔚,郑玉斯,白科,陈泳先,李文红,彭金霞

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