邹潇潇,刘鑫鑫,朱军,林勇,黄惠琴,鲍时翔

• 1:中国热带农业科学院热带生物技术研究所

摘要(Abstract)：

为了解海南省冬青叶马尾藻不同地理种群的多样性,从潭门镇和长坡镇收集到12份冬青叶马尾藻样品,通过PCR扩增和测序获得了其cox1(细胞色素C氧化酶亚基1)、rbc L(1,5-二磷酸核酮糖羧化酶/加氧酶大亚基)及ITS(内转录间隔区)序列。多序列比对显示,2个种群的冬青叶马尾藻的cox1、rbc L序列很保守,未发现多态性位点;而ITS序列变异较大,在碱基组成和序列长度上均有丰富的多态性。根据ITS的序列特征将所分析样品分为A、B和C 3个类群。系统进化分析显示,3个类群能以较高的置信度与数据库中其他地域来源的冬青叶马尾藻聚为一支,其中A、B类群的亲缘关系非常近,而C类群与其他地域来源的冬青叶马尾藻更相似。

关键词(KeyWords)： 褐藻;冬青叶马尾藻;种群;多样性;系统进化

Abstract:

Keywords:

基金项目(Foundation): 海洋公益性行业科研专项(2014050402);; 海南省自然科学基金(20163119);; 国家海洋经济创新发展区域示范项目(12PYY001SF08);; 海南省科技兴海专项(XH201408);; 海南省重点实验室和工程技术研究中心建设专项(sys2014005);; 中央级公益性科研院所基本科研业务费专项(ITBB2015RC07、1630052015038)

作者(Author): 邹潇潇,刘鑫鑫,朱军,林勇,黄惠琴,鲍时翔

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