张才,薛红莎,李玉玉,刘白平,张永平,宋采

• 1:广东海洋大学食品科技学院

摘要(Abstract)：

以慢性应激诱导的抑郁症为模型,通过测定大鼠蔗糖水偏好度、强迫游泳静止时间、肾上腺指数、血清皮质酮水平、巨噬细胞活性和吞噬能力,研究DHA的抗抑郁效果。结果表明:应激组与对照组相比,蔗糖水偏好度显著下降(P<0.05),强迫游泳静止时间显著延长(P<0.01),肾上腺指数、巨噬细胞活性和吞噬能力均显著增强(P<0.05);长期喂食DHA可以改善蔗糖水偏好度和静止时间(P<0.01),降低肾上腺指数、血清皮质酮水平、巨噬细胞活性和吞噬能力(P<0.05)。慢性应激可引起外周炎症反应增强和应激激素水平升高,DHA可在一定程度上调节外周炎症反应和激素水平,改善慢性应激诱导的抑郁症状。

关键词(KeyWords)： 抑郁症;行为;慢性不可预知性轻度应激;巨噬细胞;DHA

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81171118,81471223)

作者(Author): 张才,薛红莎,李玉玉,刘白平,张永平,宋采

参考文献(References)：

• [1]BITEW T.Prevalence and risk factors of depression in Ethiopia:a review[J].Ethiopian journal of health sciences,2014,24(2):161-169.
• [2]GONZALEZ H M,TARRAF W,WHITFIELD K E,et al.The epidemiology of major depression and ethnicity in the United States[J].J Psychiatr Res,2010,44(15):1043-1051.
• [3]FOURNIER J C,DERUBEIS R J,HOLLON S D,et al.Antidepressant drug effects and depression severity:a patient-level meta-analysis[J].Jama,2010,303(1):47-53.
• [4]VASWANI M,LINDA F K,RAMESHS.Role of selective serotonin reuptake inhibitors in psychiatric disorders:a comprehensive review[J].Progress in neuropsychopharmacology and biological psychiatry,2003,27(1):85-102.
• [5]MONTGOMERY S,BALDWIN D,RILEY A.Antidepressant medications:a review of the evidence for drug-induced sexual dysfunction[J].Journal of affective disorders,2002,69(1):119-140.
• [6]BORTOLOTTO V,CUCCURAZZU B,CANONICO P L,et al.NF-k B mediated regulation of adult hippocampal neurogenesis:relevance to mood disorders and antidepressant activity[J].Bio Med research international,2014,2014:612798.
• [7]MIURA H,OZAKI N,SAWADA M,et al.A link between stress and depression:shifts in the balance between the kynurenine and serotonin pathways of tryptophan metabolism and the etiology and pathophysiology of depression[J].Stress,2008,11(3):198-209.
• [8]WILLNER P.Chronic mild stress(CMS)revisited:consistency and behavioural-neurobiological concordance in the effects of CMS[J].Neuropsychobiology,2005,52(2):90-110.
• [9]STREKALOVA T,COUCHY,KHOLOD N,et al.Update in the methodology of the chronic stress paradigm:internal control matters[J].Behavioral and brain functions,2011,7:9.
• [10]SONG C,ZHAO S.Omega-3 fatty acid eicosapentaenoic acid.A new treatment for psychiatric and neurodegenerative diseases:a review of clinical investigations[J].Expert opinion on investigational drugs,2007,16(10):1627-1638.
• [11]SONG C,PHILLIPS A,LEONARD B,et al.Ethyl-eicosapentaenoic acid ingestion prevents corticosterone-mediated memory impairment induced by central administration of interleukin-1βin rats[J].Molecular psychiatry,2004,9(6):630-638.
• [12]BOURIN M,MOCZER E,PORSOLT R.Antidepressant-like activity of S 20098(agomelatine)in the forced swimming test in rodents:involvement of melatonin and serotonin receptors[J].Journal of psychiatry&neuroscience Jpn,2004,29(2):126.
• [13]FERRAZ A C,DELATTRE A M,ALMENDRA R G,et al.Chronicω-3 fatty acids supplementation promotes beneficial effects on anxiety,cognitive and depressive-like behaviors in rats subjected to a restraint stress protocol[J].Behavioural brain research,2011,219(1):116-122.
• [14]SU K P.Inflammation in psychopathology of depression:clinical,biological,and therapeutic implications[J].Biomedicine,2012,2(2):68-74.
• [15]ELLIS F,SANDERS T.Long chain polyunsaturated fatty acids in endogenous depression[J].Journal of Neurology,Neurosurgery&Psychiatry,1977,40(2):168-169.
• [16]SONG C,ZHANG X Y,MANKU M.Increased phospholipase A2 activity and inflammatory response but decreased nerve growth factor expression in the olfactory bulbectomized rat model of depression:effects of chronic ethyl-eicosapentaenoate treatment[J].The Journal of Neuroscience,2009,29(1):14-22.
• [17]LUCHTMAN D W,MENG Q,WANG X,et al.Omega-3 fatty acid eicospentaenoic acid attenuates MPP+‐induced neurodegeneration in fully differentiated human SH-SY5Y and primary mesencephalic cells[J].J Neurochem,2013,124(6):855-868.
• [18]黄升谋.ω-3系列多不饱和脂肪酸生理功能及其机理[J].襄樊学院学报,2010,31(5):16-19.
• [19]LUCHTMAN D W,SONG C.Cognitive enhancement by omega-3 fatty acids from child-hood to old age:findings from animal and clinical studies[J].Neuropharmacology,2013,64:550-565.
• [20]SONG C,LEONARD B E,HORROBIN D F.Dietary ethyl-eicosapentaenoic acid but not soybean oil reverses central interleukin-1-induced changes in behavior,corticosterone and immune response in rats[J].Stress,2004,7(1):43-54.
• [21]TAPAVARAPRUK P,SONG C.Reductions of acetylcholine release and nerve growth factor expression are correlated with memory impairment induced by interleukin-1beta administrations:effects of omega-3fatty acid EPA treatment[J].Journal of neurochemistry,2010,112(4):1054-1064.
• [22]SONG C,Li X,LEONARD B E,et al.Effects of dietary n-3 or n-6 fatty acids on interleukin-1beta-induced anxiety,stress and inflammatory responses in rats[J].Journal of lipid research,2003,44(10):1984-1991.
• [23]LINGE R,PAZOSá,DIAZá.Social isolation differentially affects anxiety and depressive-like responses of bulbectomized mice[J].Behavioural brain research,2013,245:1-6.
• [24]谭永振,武凯歌,张竞之,等.王氏连朴饮对脾胃湿热证大鼠行为,肾上腺指数及血清Cort的影响[J].现代生物医学进展,2012,12(14):2623-2625.
• [25]杨杰,卫东锋,王文潇,等.五指毛桃水提物对免疫抑制小鼠细胞免疫的影响[J].中药药理与临床,2015,31(6):111-114.
• [26]张莘莘,李文娟,聂少平,等.黑灵芝多糖对体外培养的小鼠腹腔巨噬细胞功能的影响[J].中国药理学通报,2010,26(9):1139-1142.
• [27]KREISEL T,FRANK M G,LICHT T,et al.Dynamic microglial alterations underlie stress-induced depressive-like behavior and suppressed neurogenesis[J].Molecular Psychiatry,2013,19(6):699-709.
• [28]AKIL H.Stressed and depressed[J].Nature medicine,2005,11(2):116-118.
• [29]SMITH R.The macrophage theory of depression[J].Med Hypotheses,1991,35(4):298-306.
• [30]李康,郭强,王翠妮,等.M1和M2型巨噬细胞表型的比较分析[J].现代免疫学,2008,28(3):177-183.
• [31]SONG C,ZHAO S.Omega-3 fatty acid eicosapentaenoic acid.A new treatment for psychiatric and neurodegenerative diseases:a review of clinical investigations[J].2007,16(10):1627-38.
• [32]DARIOS F,DAVLETOV B.Omega-3 and omega-6 fatty acids stimulate cell membrane expansion by acting on syntaxin 3[J].Nature,2006,440(7085):813-817.
• [33]SUBLETTE M E,HIBBELN J R,GALFALVY H,et al.Omega-3 polyunsaturated essential fatty acid status as a predictor of future suicide risk[J].American Journal of Psychiatry,2006,163(6):1100-1102.