魏雯璐,刘琪,张丞澍,郝瑞娟,廖永山,王庆恒,邓岳文

• 1:广东海洋大学水产学院
• 2:南方海洋科学与工程广东省实验室(湛江)
• 3:广东省珍珠养殖与加工工程技术研究中心
• 4:广东海洋大学珍珠研究所

摘要(Abstract)：

【目的】探究马氏珠母贝(Pinctada fucata martensii)对干露胁迫的响应,为马氏珠母贝健康养殖和遗传育种提供基础资料。【方法】在不同温度下对马氏珠母贝进行干露胁迫,探究温度对马氏珠母贝干露耐受的影响;筛选出马氏珠母贝干露胁迫敏感组和耐受组,比较两组的免疫相关基因表达量和植核后休养期存活率。【结果】(1)22～34℃条件下,马氏珠母贝干露耐受能力随温度升高及干露时间延长而降低;(2)22℃下,S组和T组的脯氨酸羟化酶(PHD)、葡萄糖转运蛋白(GLUT)、髓样分化因子(MYD88)、热休克蛋白(Hsp70、Hsp20)和凋亡抑制因子(cIAP)6个基因在干露过程中表达量基本呈上升趋势,组间差异显著(P<0.05);(3)植核试验结果表明耐受组植核后休养期的存活率显著高于敏感组(P <0.05)。【结论】马氏珠母贝在高温环境下干露耐受能力降低,在适温条件下耐受组比敏感组表现出较高的存活率。

关键词(KeyWords)： 马氏珠母贝;干露胁迫;温度;育珠;免疫;耐受能力

Abstract:

Keywords:

基金项目(Foundation): 广东省基础与应用基础研究基金项目(2020A1515010691和2019A1515111026);; 广东省普通高校青年创新人才类项目(2019KQNCX043);; 广东省教育厅珍珠研究创新团队(2017KCXTD016);; 广东省现代农业产业技术创新团队专项(2020KJ146)

作者(Author): 魏雯璐,刘琪,张丞澍,郝瑞娟,廖永山,王庆恒,邓岳文

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