吉薇,章超桦,吉宏武

• 1:广东海洋大学食品科技学院
• 2:广东海洋大学广东省水产品加工与安全重点实验室
• 3:广东海洋大学水产品深加工广东普通高校重点实验室

摘要(Abstract)：

采用动物蛋白水解酶酶解制备南极磷虾二肽基肽酶-IV(DPP-IV)抑制肽,以DPP-IV抑制率和可溶性蛋白含量为主要指标,考察温度、反应时间、pH、酶添加量对南极磷虾的酶解效果。在单因素的基础上,依据中心组合实验设计原理,运用4因素3水平的响应面分析法,建立动物蛋白水解酶酶解南极磷虾制备DPP-IV抑制肽的二次多项式数学模型,并以DPP-IV抑制率为响应值作响应面。结果表明:南极磷虾酶法制备DPP-IV抑制肽的最佳工艺参数为酶解时间3.85 h、温度45.02℃、pH值7.58、酶添加量237.55 U/g原料;在此条件下,酶解液DPP-IV抑制率的预测值为64.82%,验证值为64.78%,优化方法可行。

关键词(KeyWords)： 南极磷虾;DPP-IV抑制肽;响应面;工艺参数优化

Abstract:

Keywords:

基金项目(Foundation): 广东省高等学校学科建设项目(2013CXZDA020);; 广东省产学研项目(2013B090600155);; 广东海洋大学创新强校基金项目(2015)

作者(Author): 吉薇,章超桦,吉宏武

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