杨易,高静,陈彦,鲁冰,谢莉萍,张荣庆

• 1:清华大学生命科学学院

摘要(Abstract)：

【目的】研究无定型碳酸钙结合蛋白(ACCBP)基因的上游调控机制。【方法】以合浦珠母贝(Pinctada fucata)为研究对象,利用基因组步移技术克隆合浦珠母贝ACCBP基因(PfACCBP)的启动子。设计启动子截短实验,通过双荧光素酶标记法确定启动子与转录因子CREB的结合位点,定位启动子中的功能元件。克隆转录因子环腺苷效应元件结合蛋白(CREB),并通过共转染实验和凝胶迁移率分析检测启动子的相对转录活性。【结果】获得合浦珠母贝ACCBP基因的启动子序列,ACCBP启动子在HEK-293T细胞系中转录活性较高;该启动子上存在CRE元件,该元件可在转录过程中与CREB蛋白直接结合,大幅上调ACCBP启动子的转录效率。【结论】转录因子CREB可通过与ACCBP启动子上的CRE元件结合而增强转录活性的调控作用。

关键词(KeyWords)： 合浦珠母贝;无定型碳酸钙结合蛋白;环腺苷效应元件结合蛋白;启动子;转录活性

Abstract:

Keywords:

基金项目(Foundation): 清华大学研究生院基金“生物矿化细胞对珍珠层形成的作用及其调控机制”（31372502）

作者(Author): 杨易,高静,陈彦,鲁冰,谢莉萍,张荣庆

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