于辉,李华,颜其贵,林涛,冯婷,冯迎春

• 1:佛山科技学院生命科学学院
• 2:四川农业大学动物医学院

摘要(Abstract)：

通过RT-PCR方法,以草鱼、鳙鱼、鲫鱼、鲤鱼、齐口裂腹鱼等5种鲤科重要经济鱼类的垂体总RNA为模板扩增出其生长激素(fish growth hormone,fGH)的完整ORF序列,并克隆到pMD18-T载体上,命名为pMD-1(草鱼)、pMD-2(鳙鱼)、pMD-3(鲫鱼)、pMD-4(鲤鱼)、pMD-5(齐口裂腹鱼)。测序结果显示,ORF序列其长度均为633 bp。序列分析表明,所有ORF序列均以ATG为起始密码,以TAG为终止密码,编码210个氨基酸残基,推导的生长激素前体由22个氨基酸的信号肽和188个氨基酸的成熟肽组成。同源性分析表明,草鱼、鳙鱼、鲤鱼、鲫鱼和齐口裂腹鱼的fGH同源性在93.3%~99.5%之间。

关键词(KeyWords)： 鱼类生长激素(fGH);cDNA克隆;序列分析;草鱼;鳙鱼;鲫鱼;鲤鱼;齐口裂腹鱼

Abstract:

Keywords:

基金项目(Foundation): 广东省自然科学基金(7010423)

作者(Author): 于辉,李华,颜其贵,林涛,冯婷,冯迎春

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