李亚娟,王佳佳,张才,杨隆恩,古敏晴,唐海婷,宋采

• 1:广东海洋大学食品科技学院
• 2:广东海洋大学深圳研究院

摘要(Abstract)：

【目的】探究海参酶解液对高糖高脂所致的斑马鱼抑郁样行为的改善作用。【方法】将斑马鱼随机分为对照组、模型组各1组,和海参酶解液高剂量(1.0m L/L)治疗组、低剂量(0.5m L/L)治疗组各1组。每天利用高浓度葡萄糖(质量分数2%)系统浸泡及高胆固醇(质量分数10%)饲料饲喂诱导斑马鱼高糖高脂模型,高低浓度海参酶解液浸泡治疗。14 d后进行新鱼缸潜水行为实验,取鱼体匀浆进行组织各生化指标的检测,包括葡萄糖(Glucose, Glu)、总胆固醇(Total cholesterol, TC)、皮质醇(Cortisol, Cor)浓度。【结果】新鱼缸行为实验中,与对照组相比较,模型组斑马鱼的行为学实验都有明显差异,其中焦虑型行为潜伏期、冻结次数和冻结时间等抑郁型行为增加,探究型行为顶部转移和顶部时间减少,而1.0 mL/L高剂量海参酶解液可以逆转这些变化。模型组葡萄糖、总胆固醇、皮质醇浓度相比对照组显著升高,而给予海参酶解液治疗后,高剂量治疗组对模型组的葡萄糖、总胆固醇及皮质醇浓度均有显著降低的作用。【结论】海参酶解液可以通过改善高糖高脂诱导的糖尿病模型中糖脂水平来改善抑郁样行为和应激激素。

关键词(KeyWords)： 海参酶解液;高糖高脂;糖尿病;抑郁样行为

Abstract:

Keywords:

基金项目(Foundation): 深圳市大鹏新区产业发展专项资金科技扶持项目(KY20170210);研发改善记忆,抗抑郁和焦虑及防治精神和神经退行性疾病的海参活性组分产品(B16074);; 湛江市科技攻关计划项目“脑健康海洋药物与营养品重点实验室”

作者(Author): 李亚娟,王佳佳,张才,杨隆恩,古敏晴,唐海婷,宋采

参考文献(References)：

• [1]DZIEMIDOK P,MAKARA-STUDZI?S M,JAROSZM J.Diabetes and depression:a combination of civilization and life-style diseases is more than simple problem adding-literature review[J].Ann Agric Environ Med,2011,18(2):318-322.
• [2]LEONE T,COAST E,NARAYANAN S,et al.Diabetes and depression comorbidity and socio-economic status in low and middle income countries(LMICs):a mapping of the evidence[J].Globalization&Health,2012,8(1):39-49.
• [3]ROY T,LLOYD C E,PARVIN M,et al.Prevalence of co-morbid depression in out-patients with type 2 diabetes mellitus in Bangladesh[J].BMC Psychiatry,2012,12(1):123-133.
• [4]施展.斑马鱼--一只游入人类新药研发领域的小鱼[J].世界最新医学信息文摘,2016,16(20):29-30.
• [5]刘晓金,陈华利,高燕,等.斑马鱼模式研究现状及应用情况进展[J].辽宁中医药大学学报,2016,18(4):125-127.
• [6]谢静,王卫庆.斑马鱼模型和相关技术及其在内分泌系统的应用和前景[J].国际内分泌代谢杂志,2008,28(1):30-32.
• [7]张朝燕,吴文惠,刘克海,等.基于海洋药物特色的药剂学综合实验设计[J].科技资讯,2010(32):236-236.
• [8]张梅秀,王锡昌,刘源.海参生物活性研究进展[J].天然产物研究与开发,2012,24(8):1151-1159.
• [9]逄龙.海参主要活性物质对血管内皮细胞的保护作用及抗肿瘤活性的研究[D].青岛:中国海洋大学,2007.
• [10]杨隆恩,张永平,张才,等.海参酶解液对角叉菜胶所致小鼠血栓形成的抑制作用[J].广东海洋大学学报,2018,38(4):57-62.
• [11]GLEESON M,CONNAUGHTON V,ARNESON LS.Induction of hyperglycaemia in zebrafish(Danio rerio)leads to morphological changes in the retina[J].Acta Diabetologica,2007,44(3):157-163.
• [12]孙宏峰,杨晓晖.糖尿病合并抑郁症的诊断和处理[J].中华全科医学,2017,15(7):1097-1098.
• [13]LUSTMAN P J,GRIFFITH L S,CLOUSE R E.Depression in adults with diabetes.Results of 5-yr follow-up study[J].Diabetes Care,1988,11(8):605-616.
• [14]ARIGO D,SMYTH J M,HAGGERTY K,et al.The social context of the relationship between glycemic control and depressive symptoms in type 2 diabetes[J].Chronic Illness,2015,11(1):33-43.
• [15]ALMEIDA-SOUZA T H,GOES T C,TEIXEIRA-SILVAF.Pharmacological validation of the free-exploratory paradigm in male Wistar rats:A proposed test of trait anxiety[J].Pharmacology Biochemistry&Behavior,2015,135:114-120.
• [16]HUGHES R N,OTTO M T.Anxiolytic effects of environmental enrichment attenuate sex-related anxiogenic effects of scopolamine in rats[J].Progress in Neuro-psychopharmacology and Biological Psychiatry,2013,40(1):252-259.
• [17]KANGUSSU L M,ALMEIDA-SANTOS A F,BADERM,et al.Angiotensin-(1-7)attenuates the anxiety and depression-like behaviors in transgenic rats with low brain angiotensinogen[J].Behavioural Brain Research,2013,257(4):25-30.
• [18]KJ?RSG?RD I V H,JESSEN F.Proteome analysis elucidating post-mortem changes in cod(Gadus morhua)muscle proteins[J].Journal of Agricultural&Food Chemistry,2003,51(14):3985-3991.
• [19]TEROVA G,ADDIS M F,PREZIOSA E,et al.Effects of postmortem storage temperature on sea bass(Dicentrarchus labrax)muscle protein degradation:analysis by 2-D DIGE and MS[J].Proteomics,2011,11(14):2901-2910.
• [20]STEWART A,GAIKWAD S,KYZAR E,et al.Modeling anxiety using adult zebrafish:a conceptual review[J].Neuropharmacology,2012,62(1):135-143.
• [21]DOS M S,DE G M,PRESTES A S,et al.Hyperglycemia elicits anxiety-like behaviors in zebrafish:Protective role of dietary diphenyl diselenide[J].2018S0278-5846(18):30036-30044.
• [22]LUSTMAN P J,ANDERSON R J,FREEDLAND K E,et al.Depression and poor glycemic control:a meta-analytic review of the literature[J].Diabetes Care,2000,23(7):934-942.
• [23]TéLLEZ-ZENTENO J F,CARDIEL M H.Risk factors associated with depression in patients with type 2diabetes mellitus[J].Archives of Medical Research,2002,33(1):53-60.
• [24]贾杰芳,刘玉美,陈传刚,等.抑郁程度对2型糖尿病共病抑郁患者血糖波动与认知功能影响及机制分析[J].精神医学杂志,2017,30(1):45-49.
• [25]KATON W,MAJ M,SARTORIUS N.Depression and Diabetes[M].John Wiley&Sons,2010:1-27.
• [26]徐说,林文娟.抗炎性细胞因子与抑郁症[J].生物化学与生物物理进展,2014(11):1099-1108.
• [27]CHAN O,CHAN S,INOUYE K,et al.Molecular regulation of the hypothalamo-pituitary-adrenal axis in streptozotocin-induced diabetes:effects of insulin treatment[J].Endocrinology,2001,142(11):4872-4880.