李静,庞欢瑛,简纪常,鲁义善,汤菊芬,吴灶和

• 1:广东海洋大学水产学院
• 2:广东省水产经济动物病原生物学及流行病学重点实验室
• 3:广东省教育厅水产经济动物病害控制重点实验室
• 4:仲恺农业工程学院

摘要(Abstract)：

根据NCBI上登录的溶藻弧菌(Vibrio alginolyticus)全基因组序列,设计一对克隆溶藻弧菌HY9901株Ⅲ型分泌系统"分子尺"蛋白VscP全长基因的特异性引物,PCR扩增获得基因的全长片段。结果显示,vsc P(Gen Bank登录号FR780678)开放阅读框ORF为1 206 bp,共编码401个氨基酸残基。根据推导的氨基酸序列预测其分子质量约为44.4 ku,等电点为5.72。Signal 4.1 Server和TMHMM Server 2.0预测结果表明,VscP无信号肽与跨膜结构域。Soft Berry-Psite预测结果显示,VscP含有3个N端糖基化位点,2个cAMP及c GMP依赖性蛋白激酶磷酸化位点,5个蛋白激酶C磷酸化位点,9个酪蛋白激酶II磷酸化位点,2个N端豆蔻酰基化位点,1个酰胺化位点和3个微体C末端靶信号位点。运用MEGA6.0构建的VscP系统进化树显示,溶藻弧菌VscP与副溶血弧菌(V.parahaemolyticus)聚为一簇。应用SWISS-MODEL软件构建VscP亚基三维结构模型发现,其与鞭毛Fil K蛋白有相似构型。

关键词(KeyWords)： 溶藻弧菌;Ⅲ型分泌系统;基因克隆;生物信息学分析;vscP

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31402344);; 海洋经济创新发展区域示范专项(GD2012-B01004)

作者(Author): 李静,庞欢瑛,简纪常,鲁义善,汤菊芬,吴灶和

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