刘加慧,王辉,杨洪帅

• 1:广东海洋大学水产学院

摘要(Abstract)：

以小球藻(Chlorella sp.CHX-1)为实验材料,采用外切中心复合设计试验法(CCC)研究了温度(16~34℃)和盐度(15~45)对小球藻生长的联合效应。结果表明:温度与盐度的一次、二次效应对小球藻比生长速率都有极显著影响(P<0.01),温度、盐度之间的互作效应对小球藻比生长速率影响显著(P<0.05),温度的效应大于盐度的效应。响应曲面法分析发现,随着温度和盐度的升高,小球藻的比生长速率呈先上升后下降的趋势。建立小球藻生长与温度、盐度的回归模型方程,该模型的决定系数0.991 7,矫正决定系数0.985 8,表明模型的拟合度极高;模型的预测决定系数0.949 8,可用于预测小球藻比生长速率的变化。通过模型优化和验证实验,得到在温度、盐度组合为25.9℃/28.4时,小球藻比生长速率达到最大值0.652,满意度为0.981。

关键词(KeyWords)： 小球藻;温度;盐度;比生长速率;模型;优化

Abstract:

Keywords:

基金项目(Foundation): 广东省教育厅工程中心建设项目(GCZX-A0909);; 国家星火计划(2011GA780001)

作者(Author): 刘加慧,王辉,杨洪帅

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