一株H5亚型禽流感病毒血凝素基因的克隆与序列分析Cloning and Sequence Analysis of Hemagglutinin Gene of the H5 Subtype Avian Influenza Virus Isolate
高志杰,梁秋婵,刘铀,陈绍红
摘要(Abstract)：
根据GenBank中发表的H5亚型禽流感病毒HA基因序列设计2对引物,用RT-PCR方法从禽流感病毒广东分离病毒株(A/Chicken/Guangdong/1997)中扩增HA基因cDNA片段,并将其克隆至pMD-18T载体进行核苷酸序列测定。结果表明:用2对引物所扩增的片段大小分别约为1300 bp和800 bp,经序列拼接获得的HA基因cDNA长度约为1601 bp,编码533个氨基酸,与国内己发表的11个代表株的核苷酸和氨基酸序列同源性为分别为96.9%~99.9%和86.5%~93.0%;HA基因编码的氨基酸序列的系统进化树也表明A/Chicken/Guang-dong/1997、A/Goose/Huadong/01/2000、A/Ck/Hk/37.4/2002、A/Chicken/Zhoukou/2/02、A/Duck/Guangxi/53/2002、A/Duck/Fujian/01/2002等毒株处于同一进化枝,亲缘关系较近;而与A/Silly/Chicken/Hongkong/SF189/01株处于不同进化枝,亲缘关系较远。
关键词(KeyWords)： 禽流感病毒(AIV);H5亚型;HA基因;克隆;序列分析
基金项目(Foundation):
作者(Author): 高志杰,梁秋婵,刘铀,陈绍红
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