超高压结合酶解法提取鱼油工艺优化Optimization of Extraction Parameter of Fish Oil by Ultra-high Pressure Combined with Enzymatic Hydrolysis
张渊超,孙钦秀,魏帅,刘书成,吉宏武,郝记明,毛伟杰,高静
摘要(Abstract):
【目的】优化黄鳍金枪鱼(Thunnusalbacares)副产品(鱼头)提取鱼油的工艺参数。【方法】以鱼油提取率为评价指标,采用单因素实验确定最佳超高压处理压强和保压时间,采用Box-Behnken响应面试验设计优化关键酶解工艺参数。【结果】建立鱼油提取率与加酶量、液料质量比、酶解温度和酶解时间之间的回归模型,加酶量、液料质量比、酶解温度和酶解时间对鱼油提取率均有显著影响(P <0.05),影响由高到低依次为加酶量、液料质量比、酶解时间、酶解温度;当超高压处理压强200 MPa、处理时间10 min、加酶量(质量分数)为1.125%、液料质量比为1∶1、酶解温度65℃和酶解时间60min时,根据质量法计算,鱼油提取率达99.51%,与模型预测值之间差异无统计学意义(P> 0.05)。【结论】采用响应面法建立的回归模型较好地描述超高压结合酶解提取鱼油的工艺过程,优化的工艺参数可有效提高鱼油提取率。
关键词(KeyWords): 鱼油;提取工艺;提取率;超高压;酶解
基金项目(Foundation): 南方海洋科学与工程广东省实验室(湛江)(ZJW-2019-06);; 国家自然科学基金面上项目(31771997);; 广东海洋大学创新强校重大培育项目(GDOU2017052603);; 广东普通高等学校水产品加工与利用创新团队项目(GDOU201603503)
作者(Author): 张渊超,孙钦秀,魏帅,刘书成,吉宏武,郝记明,毛伟杰,高静
参考文献(References):
- [1] BABBIT K J. Intrinsic quality and species of North Pacific fish[J]. Making profits out of seafood wastes,1990, 25:25–27.
- [2] ARYEE A N, SIMPSON B K. Comparative studies on the yield and quality of solvent-extracted oil from salmon skin[J]. Journal of Food Engineering, 2009, 92(3):353-358.
- [3]方健民,黄富雄,郑钟新,等.金枪鱼的营养价值和加工利用[J].水产科技, 2006(2):8-13.
- [4] LEE H J, LEE G W, YOON I S, et al. Preparation and characterization of protein isolate from yellowfin tuna Thunnus albacares roe by isoelectric solubilization precipitation process[J]. Fisheries and Aquatic Sciences,2016, 19:1-14.
- [5]苏阳,章超桦,曹文红,等.南海产3种金枪鱼普通肉、暗色肉营养成分分析与评价[J].广东海洋大学学报,2015, 35(3):87-93.
- [6] HOUSSEM S I, JAVIER F, ESPEJO C, et al. Bi-objective optimization of tuna protein hydrolysis to produce aquaculture feed ingredients[J]. Food and Bioproducts Processing, 2019, 115:26-35.
- [7] RAJU A, MONJURUL H, BYUNG S C. Characterization of marine derived collagen extracted from the by-products of bigeye tuna(Thunnus obesus)[J]. International Journal of Biological Macromolecules, 2019, 135:668-676.
- [8] BENJAKUL S, KARNJANAPRATUM S. Characteristics and nutritional value of whole wheat cracker fortified with tuna bone bio-calcium powder[J]. Food Chemistry,2018, 259:181-187.
- [9]刘卓华,刘书成,田树良,等.硝酸银络合法浓缩金枪鱼鱼油多不饱和脂肪酸的研究[J].湛江海洋大学学报,2004, 24(4):33-37.
- [10]王乔隆,邓放明,唐春江,等.鱼油提取及精炼工艺研究进展[J].粮食与食品工业, 2008, 15(3):10-12.
- [11]刘汝萃,王彩华,肖晶,等.鱼油的提取、富集与应用研究进展[J].粮食与食品工业, 2017, 24(5):5-8.
- [12]张雅婷,王芳,曹珍珍,等.草鱼内脏油脂的提取方法及贮藏特性研究[J].食品工业科技, 2019, 40(6):121-125.
- [13]宋恭帅,张蒙娜,马永钧,等.大目金枪鱼加工副产物中鱼油提取制备及EPA分离纯化[J].核农学报,2019, 33(6):1122-1130.
- [14] ANDREA N, CARLA D P. Supercritical carbon dioxide extraction of pomegranate(Punica granatum L.)seed oil:kinetic modelling and solubility evaluation[J]. The Journal of Supercritical Fluids, 2019, 151:30-39.
- [15] RAFAELY N L, ADAUTO S, RIBEIRO, et al.Extraction from leaves of piper klotzschianum using supercritical carbon dioxide and co-solvents[J]. The Journal of Supercritical Fluids, 2019, 147:205-212.
- [16] ADELINE F V, NELLY R C. Estimation of mass transfer coefficients of the extract-ion process of essential oil from orange peel using microwave assisted extraction[J].Journal of Food Engineering, 2016, 170:136-143.
- [17]刘书成,章超桦,洪鹏志,等.酶解法从黄鳍金枪鱼鱼头中提取鱼油的研究[J].福建水产, 2007(1):46-50.
- [18]洪鹏志,刘书成,章超桦,等.酶解法提取鱼油的工艺参数优化[J].湛江海洋大学学报, 2006, 26(3):56-60.
- [19]邓倩琳.超高压联合CO2对凡纳滨对虾的保鲜效果研究[D].湛江:广东海洋大学, 2017.
- [20]李新原,李斌,颜廷才,等.超高压与超声波对蓝靛果多酚提取及抗氧化活性的影响[J].食品科学, 2017,38(2):271-277.
- [21] GB/T 5009.6-2016食品安全国家标准食品中脂肪的测定[S].北京:中国标准出版社, 2016.
- [22]尹琳琳,陈银玲,刘萍.苋菜红色素的超高压提取工艺及稳定性研究[J].食品科技, 2018, 43(9):314-320.
- [23]魏炜,李彦伟,刘凤霞,等.响应面法优化超高压提取黄静多糖工艺[J].精细化工, 2019, 36(5):875-881.
- [24]中华人民共和国农业部. SC/T 3502-2016中华人民共和国水产行业标准:鱼油[S].北京:中国农业出版社,2017.
- [25]董安迪,罗帅,刘书成,等.高密度CO2纵向溶解和扩散对虾肉糜凝胶强度的影响[J].广东海洋大学学报,2018, 38(5):57-63.
- [26] MIYASHITA K, UEMURA M, HOSOKAWA, M.Effective prevention of oxidative deterioration of fish oil:Focus on flavor deterioration[J]. Annual review of food science and technology, 2018, 9:209-226