低盐胁迫对钝吻黄盖鲽幼鱼鳃Na+/K+-ATP酶、肝脏抗氧化酶和非特异免疫酶的影响Influence of Low Salinity Stress on the Gill Na+/K+-ATPase, Liver Antioxidase and Non-Specific Immune Enzyme in Juvenile Pleuronectes yokohama
崔前进,陈冰,邱丽华,付旭,杨博学,韩雨哲,姜晨
摘要(Abstract):
研究钝吻黄盖鲽(Pleuronectes yokohama)幼鱼鳃的Na~+/K~+-ATP酶(NKA)和肝脏的超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)在低盐(6、18,分别记作S6、S18组)胁迫下的变化规律,探讨其适应盐度的规律,以及抗氧化机制和非特异免疫能力。结果显示,S6组和S18组鳃的NKA活力均呈先增后减的变化趋势,1 d时最大(P<0.05),S6组7 d时开始下降,而S18组在3 d时开始下降。肝脏的SOD和CAT活力均先升后降,其中SOD活力S6和S18组均于7 d时最大(P<0.05),14 d时S6组活力较S18组大;CAT活力S6和S18组均于7 d时最大(P<0.05),在14 d时降至初始水平。肝脏ACP活力S6、S18组均于3 d时升至最高(P<0.05),14 d时S18组降至对照组水平,而S6组则低于对照组(P<0.05);AKP活力S6和S18组均呈现先升后降的变化,在3 d达到峰值,14 d时仍高于对照组(P<0.05)。低盐胁迫影响钝吻黄盖鲽幼鱼的NKA、抗氧化酶和非特异免疫酶,S18组对盐度胁迫的适应能力更大。
关键词(KeyWords): 钝吻黄盖鲽;盐度;Na+/K+-ATP酶;抗氧化酶;非特异免疫
基金项目(Foundation): 广东省生态环境重点实验室开放基金(LFE-2016-4)
作者(Author): 崔前进,陈冰,邱丽华,付旭,杨博学,韩雨哲,姜晨
参考文献(References):
- [1]李思忠,王惠民.中国动物志:硬骨鱼纲鲽形目[M].北京:科学出版社,1995:214-216.
- [2]张岩,肖永双,高天翔,等.钝吻黄盖鲽野生群体遗传多样性分析[J].水产学报,2008,32(3):492-496.
- [3]毕庶万,郑振虎,刘锦华,等.几个生态因子对黄盖鲽的影响[J].生态学杂志,1989,8(5):19-21;封底.
- [4]喻子牛,孔晓瑜,谢宗墉.山东近海21种经济鱼类的核型研究[J].中国水产科学,1995,2(2):1-6.
- [5]SYASINA I G,SOKOLOVSKII A S.Assessment of the state of flounders from Sivuch’ya bight(Peter the Great Bay,Sea of Japan)based on histopathological parameters[J].Russian Journal of Marine Biology,2001,27(2):80-86.
- [6]陈雄风,蒋耀青.鱼类抗冻蛋白的研究Ⅰ.黄盖鲽血清抗冻蛋白的特性及分离[J].遗传学报,1989,16(5):367-373.
- [7]施钢,张健东,潘传豪,等.盐度渐变和骤变对褐点石斑鱼存活和摄饵的影响[J].广东海洋大学学报,2011,31(1):45-51.
- [8]姬广闻.盐度对香鱼仔鱼生长和成活率的影响[J].淡水渔业,2003,33(4):3-5.
- [9]焦海峰,尤仲杰,竺俊全,等.嘉庚蛸对温度、盐度的耐受性试验[J].水产科学,2004,23(9):7-10.
- [10]孙鹏,尹飞,彭士明,等.盐度对条石鲷幼鱼肝脏抗氧化酶活力的影响[J].海洋渔业,2010,32(2):154-159.
- [11]IMSLAND A K,GúSTAVSSON A,GUNNARSSON S,et al.Effects of reduced salinities on growth,feed conversion efficiency and blood physiology of juvenile Atlantic halibut(Hippoglossus hippoglossus L.)[J].Aquaculture,2008,274(2/3/4):254-259.
- [12]强俊,任洪涛,徐跑,等.温度与盐度对吉富品系尼罗罗非鱼幼鱼生长和肝脏抗氧化酶活力的协同影响[J].应用生态学报,2012,23(1):255-263.
- [13]SHI Z H,HUANG X X,FU R B,et al.Salinity stress on embryos and early larval stages of the pomfret Pampus punctatissimus[J].Aquaculture,2008,275(1/2/3/4):306-310.
- [14]HUANG C Y,CHAO P L,LIN H C.Na+/K+-ATPase and vacuolar-type H+-ATPase in the gills of the aquatic air-breathing fish Trichogaster microlepis in response to salinity variation[J].Comparative Biochemistry and Physiology Part A:Molecular&Integrative Physiology,2010,155(3):309-318.
- [15]ROMBOUGH P.The functional ontogeny of the teleost gill:which comes first,gas or ion exchange?[J].Comparative Biochemistry and Physiology Part A:Molecular&Integrative Physiology,2007,148(4):732-742.
- [16]SINGER T D,KEIR K R,HINTON M.Structure and regulation of the cystic fibrosis transmembrane conductance regulator(CFTR)gene in killifish:a comparative genomics approach[J].Comparative Biochemistry and Physiology Part D:Genomics and Proteomics,2008,3(2):172-85.
- [17]王涛,苗亮,李明云,等.突降盐度胁迫对大黄鱼(Pseudosciaena crocea)血清生理生化及鳃丝Na+/K+-ATP酶活性的影响[J].海洋与湖沼,2013,44(2):421-426.
- [18]郭黎,马爱军,王新安,等.盐度和温度对大菱鲆幼鱼抗氧化酶活性的影响[J].大连海洋大学学报,2012,27(5):422-428.
- [19]边平江,邱成功,徐善良,等.盐度对暗纹东方鲀生长、非特异性免疫和抗氧化酶活力的影响[J].水生生物学报,2014,38(1):108-114.
- [20]BRADSHAW D,MCCORMICK S.Hormonal control of salt and water balance in vertebrates—a symposium[J].General and Comparative Endocrinology,2006,147(1):1-2.
- [21]EVANS D H,PIERMARINI P M,CHOE K P.The multifunctional fish gill:dominant site of gas exchange,osmoregulation,acid-base regulation,and excretion of nitrogenous waste[J].Physiological Reviews,2005,85(1):97-177.
- [22]HWANG P P,LEE T H,LIN L Y.Ion regulation in fish gills:recent progress in the cellular and molecular mechanisms[J].American Journal of Physiology–Regulatory,Integrative and Comparative Physiology,2011,301(1):R28-R47.
- [23]HWANG P P,SUN C M,WU S M.Changes of plasma osmolality,chloride concentration and gill Na-K-ATPase activity in tilapia Oreochromis mossambicus during seawater acclimation[J].Marine Biology,1989,100(3):295-299.
- [24]MADSEN S S,LARSEN B K,JENSEN F B.Effects of freshwater to seawater transfer on osmoregulation,acid-base balance and respiration in river migrating whitefish(Coregonus lavaretus)[J].Journal ofComparative Physiology B,1996,166(2):101-109.
- [25]潘鲁青,唐贤明,刘泓宇,等.盐度对褐牙鲆(Paralichthys olivaceus)幼鱼血浆渗透压和鳃丝Na+-K+-ATPase活力的影响[J].海洋与湖沼,2006,37(1):1-6.
- [26]OTTO D M E,MOON T W.Endogenous antioxidant systems of two teleost fish,the rainbow trout and the black bullhead,and the effect of age[J].Fish Physiology and Biochemistry,1996,15(4):349-358.
- [27]FILHO D W,GIULIVI C,BOVERIS A.Antioxidant defences in marine fish—I.Teleosts[J].Comparative Biochemistry and Physiology Part C:Pharmacology,Toxicology and Endocrinology,1993,106(2):409-413.
- [28]B?UF G,PAYAN P.How should salinity influence fish growth?[J].Comparative Biochemistry and Physiology Part C:Toxicology&Pharmacology,2001,130(4):411-423.
- [29]ANSALDO M,LUQUET C M,EVELSON P A,et al.Antioxidant levels from different Antarctic fish caught around South Georgia Island and Shag Rocks[J].Polar Biology,2000,23(3):160-165.
- [30]王素久,张海发,赵俊,等.不同盐度对斜带石斑鱼幼鱼生长和生理的影响[J].广东海洋大学学报,2011,31(6):39-44.
- [31]杨健,陈刚,黄建盛,等.温度和盐度对军曹鱼幼鱼生长与抗氧化酶活性的影响[J].广东海洋大学学报,2007,27(4):25-29.
- [32]唐贤明,隋曌,田景波,等.盐度对大菱鲆幼鱼耗氧率和排氨率的影响[J].南方水产,2006,2(4):54-58.
- [33]王晓杰,张秀梅,李文涛.盐度胁迫对许氏平鲉血液免疫酶活力的影响[J].渔业科学进展,2005,26(6):17-21.
- [34]AN K W,KIM N N,SHIN H S,et al.Profiles of antioxidant gene expression and physiological changes by thermal and hypoosmotic stresses in black porgy(Acanthopagrus schlegeli)[J].Comparative Biochemistry and Physiology Part A:Molecular&Integrative Physiology,2010,156(2):262-268.
- [35]张晨捷,张艳亮,高权新,等.低盐胁迫对黄姑鱼幼鱼肝脏抗氧化功能的影响[J].南方水产科学,2015,11(4):59-64.
- [36]郭勤单,王有基,吕为群.温度和盐度对褐牙鲆幼鱼渗透生理及抗氧化水平的影响[J].水生生物学报,2014,38(1):58-67.
- [37]王妤,庄平,章龙珍,等.盐度对点篮子鱼的存活、生长及抗氧化防御系统的影响[J].水产学报,2011,35(1):66-73.
- [38]艾春香,陈立侨,高露姣,等.VC对河蟹血清和组织中超氧化物歧化酶及磷酸酶活性的影响[J].台湾海峡,2002,21(4):431-438.
- [39]张龙岗,安丽,孙栋,等.盐度对澳洲宝石鲈幼鱼3个免疫因子活力的影响[J].水生态学杂志,2011,32(6):110-114.
- [40]冯娟,徐力文,林黑着,等.盐度变化对军曹鱼稚鱼相关免疫因子及其生长的影响[J].中国水产科学,2007,14(1):120-125.