一株海水氨氧化细菌的系统发育分析Phylogenetic Analysis of a Strain of Marine Ammonia-Oxidizing Bacterium
黄建军,简纪常,吴灶和,丁燏
摘要(Abstract)：
应用PCR技术扩增16S rRNA基因和amoA(ammonia monooxygenase subunit A)的基因片段,并测定其序列,对一株源于海水养殖水体的高效氨氧化细菌(ammonia-oxidizing bacteria,AOB)进行了系统发育分析。结果表明,经PCR扩增得到了1 098 bp的16S rRNA基因片段和491 bp的amoA基因片段,将其序列用NCBI-Blast软件在GenBank数据库中进行同源性检索后发现,该菌株的16S rRNA基因序列和amoA基因序列分别与亚硝化单胞菌Nitrosomonas sp.NS20的相对应基因片段相似性分别为98.4%和96.7%。在此基础上构建了系统发育树,表明用该菌株的16S rRNA基因片段和amoA基因片段构建的系统发育树均与亚硝化单胞菌属类聚在一起,结合该菌株形态和生理生化特性,鉴定该株氨氧化细菌属亚硝化单胞菌。
关键词(KeyWords)： 氨氧化细菌;16SrRNA;amoA;聚合酶链反应;同源性分析
基金项目(Foundation): 广东省科技计划项目(2003C20332和2005B20301004)
作者(Author): 黄建军,简纪常,吴灶和,丁燏
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