李亚俐,田尹琼,石柳,吴文锦,陈胜,陈朗,郭晓嘉,熊光权,汪兰,孙智达

• 1:华中农业大学食品科技学院
• 2:农业农村部农产品冷链物流技术重点实验室
• 3:湖北省农业科学院农产品加工与核农技术研究所
• 4:湖北工业大学生命科学与健康工程学院

摘要(Abstract)：

【目的】研究冷藏斑点叉尾鮰（Ictalurus punctatus）的主要腐败菌及其产生物胺的能力，为冷鲜鱼片保鲜、防腐及安全食用提供参考。【方法】从冷藏斑点叉尾鮰中分离并鉴定7株腐败菌，测定7株腐败菌的胞外蛋白酶活性、生物胺的产量评估腐败菌的产生物胺能力。【结果与结论】从冷藏鱼片中分离出7株腐败菌NA-1、VRBA-1、STAA-1、NA-2、IA-2、CFC-1、IA-1，分别鉴定为哈夫尼亚菌（Hafniasp.）、沙雷氏菌（Serratiasp.）、热杀索丝菌（Brochothrix thermosphacta）、杀鲑气单胞菌（Aeromonas salmonicida）、波罗的海希瓦氏菌（Shewanella baltica）、莓实假单胞菌（Pseudomonas fragi）、腐败希瓦氏菌（Shewanella putrefaciens）。7种腐败菌中，NA-2的蛋白水解酶活性最高，为74.89 U/mL，其次是IA-1、STAA-1和VRBA-1，分别为（65.30±1.86）、（62.03±2.15）、（60.88±1.70）U/mL。在添加前体氨基酸的LB培养基中，菌株NA-1、VRBA-1和IA-1产生物胺能力最强，腐胺产量分别为（957.38±18.15）、（1005.273±30.95）、（659.673±14.50）mg/L，尸胺产量分别为（37.033±2.36）、（14.243±1.35）、（23.863±0.66）mg/L。

关键词(KeyWords)： 斑点叉尾鮰;腐败菌;分离鉴定;生物胺;腐胺;尸胺

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系专项资金（CARS-46）;; 湖北省自然科学基金杰青项目（2022CFA095）

作者(Author): 李亚俐,田尹琼,石柳,吴文锦,陈胜,陈朗,郭晓嘉,熊光权,汪兰,孙智达

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