杨敏志;李长玲;黄翔鹄;王清珍;陈爱珊;

• 1:广东海洋大学深圳研究院
• 2:广东海洋大学水产学院
• 3:广东省藻类养殖及应用工程技术研究中心

摘要(Abstract)：

【目的】探索波吉卵囊藻(Oocystisborgei)的繁殖模式,调控其生殖过程以实现藻生物质稳定增长。【方法】在荧光显微镜下观察波吉卵囊藻似亲孢子形成过程,探究温度、照度、氮浓度对其繁殖模式的影响。【结果】波吉卵囊藻以2、4和8似亲孢子型模式繁殖,在藻细胞第二轮分裂过程中因分裂不同步,有时也形成3个似亲孢子。氮限制和弱光显著影响波吉卵囊藻繁殖模式(P <0.05)。通常情况下,波吉卵囊藻以4似亲孢子型繁殖模式繁殖,但在缺氮和弱光下2和3似亲孢子型模式的频率上升。在15～35℃温度范围内,波吉卵囊藻繁殖模式变化不大。【结论】波吉卵囊藻有4种繁殖模式。在不良环境中,2和3似亲孢子型模式频率上升。

关键词(KeyWords)： 波吉卵囊藻;似亲孢子;繁殖模式

Abstract:

Keywords:

基金项目(Foundation): 活性微藻制品产业化关键技术的研究与示范(KY20180112);广东海洋大学博士启动项目

作者(Authors): 杨敏志;李长玲;黄翔鹄;王清珍;陈爱珊;

参考文献(References)：

• [1] TAMIYA H. Correlation between photosynthesis and light-independent metabolism in the growth of Chlorella[J]. Biochimica et Biophysica Acta, 1953,12(2):23-34.
• [2] HOWARD A, PELC S R. Synthesis of desoxyribonucleic acid in normal and irradiated cells and its relation to chromosome breakage[J]. International Journal of Radiation Biology, 1986, 49(2):207-218.
• [3] LIEN T, KNUTSEN G. Phosphate as a control factor in cell division of Chlamydomonas reinhardti:studied in synchronous culture[J]. Experimental Cell Research, 1973,78(1):79-88.
• [4] LORENZEN H. Time measurements in unicellular algae and their influence on productivity[J]. Algae Biomass:Production and Use, 1980,15(3):411-419.
• [5]?ETLíK I, BERKOVáE, DOUCHA J, et al. The coupling of synthetic and reproduction processes in Scenedesmus quadricauda[J]. Arch Hydrobiol Algolog Stud, 1972, 7:172-217.
• [6] TAMIYA H. Synchronous cultures of algae[J]. Annual Review of Plant Physiology, 1966, 17(1):1-27.
• [7] NE?AS J. Influence of light on the autospore number of some chlorococcal algae[J]. Biologia Plantarum, 1969,11(6):465-469.
• [8] YAMAGISHI T, YAMAGUCHI H, SUZUKI S, et al. Cell reproductive patterns in the green alga Pseudokirchneriella subcapitata(=Selenastrum capricornutum)and their variations under exposure to the typical toxicants potassium dichromate and 3, 5-DCP[J]. PLo S One, 2017, 2(2):1-12.
• [9]胡鸿钧,魏印心.中国淡水藻类——系统、分类及生态[M].北京:科学出版社, 2006:658-670.
• [10]黄翔鹄,冯奕成,李长玲,等.虾池微藻定向培育及其对养殖环境因子的影响[J].广东海洋大学学报,2005, 25(6):25-30.
• [11] LIU M, HUANG X H, ZHANG R, et al. Uptake of urea nitrogen by Oocystis borgei in prawn(Litopenaeus vannamei)aquaculture ponds[J]. Bulletin of Environmental Contamination and Toxicology, 2018,101(5):586-591.
• [12] CHUNTAPA B, POWTONGSOOK S, MENASVETA P.Water quality control using Spirulina platensis in shrimp culture tanks[J]. Aquaculture, 2003, 220(1-4):355-366.
• [13] SNOW J W. The plankton algae of Lake Erie, with special reference to the Chlorophyceae[J]. US Fish Com Bull, 1902, 24(2):371-394.
• [14] GUILLARD R R, RYTHER J H. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea(cleve)Gran[J]. Can J Microbiol,1962, 8(2):229-239.
• [15] GUILLARD R R L, GUILLARD R, GUILLARD R, et al. Culture of phytoplankton for feeding marine invertebrates[M] Culture of Marine Invertebrate Animals.New York:Springer, 1975:29-60.
• [16] BOROWITZKA M A, BEARDALL J, RAVEN J A. The physiology of microalgae[M]. Switzerland:Springer International Publishing, 2016:4-10
• [17] MANDALAM R K, PALSSON B O. Cell cycle of Chlorella vulgaris can deviate from the synchronous binary division model[J]. Biotechnology Letters, 1997,19(6):587-591.
• [18] RIOBOO C, O’CONNOR J E, PRADO R, et al. Cell proliferation alterations in Chlorella cells under stress conditions[J]. Aquatic Toxicology, 2009, 94(3):229-237.
• [19] OLSON R J, CHISHOLM S W. Effects of light and nitrogen limitation on the cell cycle of the dinoflagellate Amphidinium carteri[J]. Journal of Plankton Research,1986, 8(4):785-793.
• [20] VAULOT D, OLSON R J, MERKEL S, et al. Cell-cycle response to nutrient starvation in two phytoplankton species, Thalassiosira weissflogii and Hymenomonas carterae[J]. Marine Biology, 1987, 95(4):625-630.
• [21] NELLE R, TISCHNER R, LORENZEN H, et al.Correlation between pigment systems and photosynthetic activity during the developmental cycle of Chlorella[J]. Biochemie und Physiologie der Pflanzen,1975, 167(6):463-472.
• [22] PRISON A, LORENZEN H. Synchronized dividing algae[J]. Annual Review of Plant Physiology, 1966,17(1):439-458.
• [23] TAMIYA H. Synchronous cultures of algae[J]. Annual Review of Plant Physiology, 1966, 17(1):1-27.
• [24] WANKA F, MULDERS P F M. The effect of light on DNA synthesis and related processes in synchronous cultures of Chlorella[J]. Archiv für Mikrobiologie, 1967,58(3):257-269.
• [25] WANKA F. Ultrastructural changes during normal and colchicine-inhibited cell division of Chlorella[J].Protoplasma, 1968, 66(1/2):105-130.
• [26] WANKA F, AELEN J M A. The effect of light on RNA and nucleotide synthesis in synchronous cultures of Chlorella[J]. Plant Science Letters, 1973, 1(4):129-135.
• [27] ZACHLEDER V, VAN DEN ENDE H. Cell cycle events in the green alga Chlamydomonas eugametos and their control by environmental factors[J]. J Cell Sci, 1992,102(3):469-474.
• [28] VíTOVáM, BI?OVáK, HLAVOVáM, et al.Chlamydomonas reinhardtii:Duration of its cell cycle and phases at growth rates affected by temperature[J].Planta, 2011, 234(3):599-608.
• [29] MORIMURA Y. Synchronous culture of Chlorella:I.Kinetic analysis of the life cycle of Chlorella ellipsoidea as affected by changes of temperature and light intensity[J]. Plant and Cell Physiology, 1959, 1(1):49-62.
• [30] VíTOVáM, ZACHLEDER V. Points of commitment to reproductive events as a tool for analysis of the cell cycle in synchronous cultures of algae[J]. Folia Microbiologica, 2005, 50(2):141.
• [31]黄翔鹄,李长玲,刘楚吾等.波吉卵囊藻培养的生态条件[J].湛江海洋大学学报, 2002, 22(3):8-12.