吴江,康恺,苑麟勇,王瑞阳,李光辉,效梅,安立龙

• 1:广东海洋大学农学院

摘要(Abstract)：

【目的】探讨不同血清和细胞因子等培养液添加物对尼罗罗非鱼(Oreochromisniloticus)精原干细胞(Spermatogonial stem cells,SSCs)生长增殖的影响。【方法】无菌取发育第Ⅲ期的尼罗罗非鱼精巢组织,用酶消化法结合差速贴壁法获得尼罗罗非鱼SSCs,在有支持细胞饲养层条件下,L-15培养液中分别添加体积分数2%、5%、10%新生牛血清(New bovine serum,NBS),体积分数1%、2%、3%罗非鱼血清以及5、10、15 ng/mL白血病抑制因子(Leukemia inhibitor factor,LIF),比较不同添加物对尼罗罗非鱼SSCs生长增殖的影响。【结果】在支持细胞饲养层上,SSCs分裂指数明显高于无饲养层的SSCs,支持细胞饲养层明显促进精原干细胞分裂增殖(P <0.01);与2%、10%NBS组相比,添加体积分数5%NBS可显著促进精原干细胞增殖(P <0.01);添加体积分数1%、2%、3%尼罗罗非鱼血清对SSCs无显著的促增殖作用(P> 0.05);添加5、10 ng/mL LIF可促进SSCs增殖,添加10 ng/mL LIF效果更佳,添加15 ng/mL LIF则抑制SSCs增殖。【结论】使用尼罗罗非鱼精巢支持细胞作饲养层,用添加体积分数5%NBS及10 ng/mL LIF的L-15培养液培养,有助于促进尼罗罗非鱼精原干细胞生长增殖。

关键词(KeyWords)： 尼罗罗非鱼;精原干细胞;添加物;生长;增殖

Abstract:

Keywords:

基金项目(Foundation): 广东省教育厅科研处青年创新人才项目(2016KQNCX062,2017KQNCX087);; 广东海洋大学博士启动基金(R17027,R17021);; 广东省大学生创新实验项目(CXXL2017050,CXXL2016012,CXXL2015010)

作者(Author): 吴江,康恺,苑麟勇,王瑞阳,李光辉,效梅,安立龙

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