梁鹏,刘雪梅,杨萍,周春霞,洪鹏志

• 1:广东海洋大学食品科技学院

摘要(Abstract)：

【目的】分析罗非鱼鱼皮多肽(TSP)对H_2O_2诱导HaCaT细胞氧化损伤的保护作用及其作用机制。【方法】将HaCaT细胞随机分成空白组(细胞正常培养)、对照组(800μmol/L的H_2O_2作用24 h)和3个罗非鱼鱼皮多肽浓度组(10、20、50μmol/L TSP),应用MTT比色法检测细胞活力,DCFH-DA法检测细胞内活性氧(ROS)含量,蛋白质印迹法测超氧化物歧化酶(SOD)和谷氨酰转肽酶(GGT)含量。【结果】与空白组相比,对照组的细胞活力明显下降(P﹤0.05),细胞内ROS含量增强,GGT表达量增加,SOD表达量减少;与对照组相比,随着多肽组浓度增加,细胞活力增强,细胞内ROS含量减少,GGT表达量减少,SOD表达量增加。【结论】10~50μmol/L罗非鱼皮多肽TSP能够提高细胞活力,通过清除细胞内的ROS,抑制GGT的表达,增加SOD的表达,达到保护H_2O_2诱导的HaCaT细胞的氧化应激损伤的效果。

关键词(KeyWords)： 罗非鱼鱼皮多肽;HaCaT细胞;氧化应激;免疫荧光

Abstract:

Keywords:

基金项目(Foundation): 广东普通高等学校水产品高值化加工与利用创新团队(GDOU2016030503);; 省部产学研合作专项(2013A090100009)

作者(Author): 梁鹏,刘雪梅,杨萍,周春霞,洪鹏志

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