海参性腺酶解物制备及其体外清除自由基活性测定Enzymatic Hydrolysis and Antioxidant Activity of Sea Cucumber Gonad
吴国宏,熊何健,李露
摘要(Abstract)：
【目的】酶解海参性腺并分析其体外清除自由基活性。【方法】采用外源性蛋白酶水解工艺制备海参性腺酶解产物,通过酶解效果比较分析,考察其体外清除DPPH自由基和羟自由基能力。【结果】确定木瓜蛋白酶为水解用酶,利用响应面优化实验确定酶解条件为酶解时间14 h、酶添加量3 000 U/g、料液比1∶40;酶解液灭酶后经离心、浓缩、干燥后得到粉状海参性腺酶解物,其主要成分质量分数分别为蛋白肽48.4%、矿物质16.8%、粗脂肪12.4%、水分9.8%;酶解产物清除DPPH自由基和羟自由基的IC50值分别为3.39、5.83 mg/mL。【结论】海参性腺酶解物具有良好的抗氧化活性。
关键词(KeyWords)： 海参性腺;木瓜蛋白酶;酶解;抗氧化
基金项目(Foundation): 福建省中青年教师教育科研项目(JT180287);; 厦门市科技计划项目(3502Z20153014);; 国家海洋局海洋公益性行业科研专项(201405016)
作者(Author): 吴国宏,熊何健,李露
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