荆鹏华,赵飞,谭爱萍,邓玉婷,邵蓬,朱雪晴,赖迎迢,巩华,黄志斌

• 1:天津农学院水产学院
• 2:中国水产科学研究院珠江水产研究所/农业农村部渔用药物创制重点实验室/广东省水产动物免疫与绿色养殖重点实验室

摘要(Abstract)：

【目的】克隆大口黑鲈（Micropterus salmoides）干扰素调节因子3(interferon regulatory factor 3,IRF3)基因，命名为MsIRF3，探究该基因在大口黑鲈先天性免疫防御中的重要作用。【方法】根据大口黑鲈基因组中IRF3基因序列设计引物，克隆MsIRF3基因；用生物信息学方法分析MsIRF3的结构特征和理化性质；用鰤诺卡氏菌（Nocardia seriolae）或维氏气单胞菌（Aeromonas veronii）感染大口黑鲈，并用病原类似物脂多糖（LPS）、脂磷壁酸（LTA）和聚肌胞苷酸[Poly(I:C)]刺激大口黑鲈头肾白细胞，采用荧光定量PCR方法检测MsIRF3基因表达量的动态变化。【结果】MsIRF3的ORF全长为1 404 bp，编码467个氨基酸。MsIRF3包含DNA结合结构域（DBD）、IRF关联结构域（IAD）及丝氨酸结构域（SRD）等3个典型结构域。同源性分析表明，MsIRF3与斜带石斑鱼（Epinephelus coioides）IRF3的亲缘性最近。qRT-PCR分析表明，MsIRF3在健康大口黑鲈各组织中广泛表达，在肠和鳃中表达量相对较高（P <0.05）。鰤诺卡氏菌或维氏气单胞菌感染后，MsIRF3在肝脏、头肾和脾中表达量均上调（P <0.01）。LPS、LTA和Poly(I:C)刺激后，大口黑鲈头肾白细胞中MsIRF3均被诱导表达。【结论】MsIRF3基因在大口黑鲈防御病原体感染的免疫应答中发挥重要作用。

关键词(KeyWords)： 大口黑鲈;干扰素调节基因3;鰤诺卡氏菌;维氏气单胞菌;免疫防御

Abstract:

Keywords:

基金项目(Foundation): 中国水产科学研究院中央级公益性科研院所基本科研业务费专项（2022GH04,2021SJ-XT3,2020TD45）;; 广州市农业和社会发展科技专题（2023B03J1305）;; 佛山市南海区农业农村局政府采购项目（2967855）

作者(Author): 荆鹏华,赵飞,谭爱萍,邓玉婷,邵蓬,朱雪晴,赖迎迢,巩华,黄志斌

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