程海燕,庞欢瑛,鲁义善,简纪常,吴灶和,蔡双虎

• 1:广东海洋大学水产学院
• 2:广东省水产经济动物病原生物学及流行病学重点实验室
• 3:广东省普通高等学校水产经济动物病害控制重点实验室
• 4:仲恺农业工程学院

摘要(Abstract)：

溶藻弧菌引起海水养殖产业弧菌病的主要病原,附着定植因子基因acfA是溶藻弧菌重要毒力基因之一,其表达产物是溶藻弧菌有效定植在宿主肠道上所必需的蛋白。根据弧菌属细菌acfA基因基因保守区设计引物,采用Touchdown PCR扩增acfA基因部分序列,Inverse PCR和Nested PCR扩增已知序列侧翼序列,成功克隆了溶藻弧菌acfA基因全长。克隆的acfA基因序列全长为743 bp,开放阅读框长度为648 bp组成,共编码215个氨基酸,在5′端上游未发现-35区和-10区序列。演绎的ACFA蛋白N端有18个氨基酸信号肽序列,表明该蛋白是分泌型蛋白;蛋白结构分析表明主要由α螺旋和不规则卷曲构成。BLAST分析表明,该蛋白氨基酸序列与其他弧菌相应蛋白同源性较高,是较保守的外膜蛋白。

关键词(KeyWords)： 溶藻弧菌,acfA基因;克隆;蛋白结构

Abstract:

Keywords:

基金项目(Foundation): 广东省教育厅育苗工程项目(2012LYM_0072)

作者(Author): 程海燕,庞欢瑛,鲁义善,简纪常,吴灶和,蔡双虎

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