周玉兰,闫守泉,牛艳茹,何惠娟,王家丰

• 1:广东医科大学附属医院临床医学研究中心
• 2:广东医科大学附属医院干细胞研发与临床转化中心

摘要(Abstract)：

利用高通量二代测序联合先进的高分辨率熔解曲线技术,在牡蛎HSP70基因中开发并验证SNP标记,获43个能够分型的SNP标记,其中33个转换类型SNP,10个颠换类型SNP标记。随机挑选8个SNP位点在大连及厦门共计116个样本中进行分型分析,8个标记位点均符合H-W平衡,最小等位基因MAF在0.037 3~0.495 7之间。有2个标记基因型在不同地域群体间具有显著差异,O_H70-20标记的T等位基因在南方群体中的频率更高,表现出更高温度耐受性(P=0.025,OR=1.809,95%CI:1.074~3.044)。O_H70-41位点T等位基因频率在大连群体中明显高于厦门群体(P=0.003,OR=0.394,95%CI:0.210~0.733),该位点的T等位基因携带个体则表现出低温耐受性。NGS-HRM策略为牡蛎SNP标记开发提供简洁、高效的方法。

关键词(KeyWords)： 太平洋牡蛎;HSP70;二代测序;高分辨率熔解曲线;单核苷酸多态性标记

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(81600445);; 广东医学院附属医院博士科研基金(528B20150012);; 广东医科大学科研基金:“两种巨蛎属牡蛎(C.zhanjiangensis和C.dianbaiensis)的物种鉴定方法开发”

作者(Author): 周玉兰,闫守泉,牛艳茹,何惠娟,王家丰

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