建鲤与黄河鲤的RAPD分子标记及其杂交优势的遗传分析RAPD Molecular Markers Between Jian Carp and Huanghe Carp and Genetic Analysis of Heterosis
佟雪红,董在杰,缪为民,袁新华,王建新,岳春梅,林连升
摘要(Abstract)：
利用RAPD技术对建鲤、黄河鲤及其杂交子代进行了遗传分析,筛选的33个引物共扩增出155条带,其中多态性片段为105条,片段S18-1600、S472-300具有种的特异性,可作为鉴别建鲤与黄河鲤的分子遗传标记。建鲤、黄河鲤及其正、反交子代群体内的遗传相似系数分别为0.8240、0.7921、0.7920、0.8569,黄河鲤与正交F1具有较高的遗传变异水平,反交F1变异最小。正交F1、反交F1与亲本(建鲤、黄河鲤)的遗传距离分别为0.2233、0.2436、0.1749、0.2026,说明子代均继承了较多的建鲤的遗传物质。分析了子代与亲本的RAPD标记类型以及各类型的条带数。应用PIT标记系统测定了子代的杂种优势并探讨了优势产生的遗传机理。
关键词(KeyWords)： 建鲤;黄河鲤;随机扩增多态性DNA(RAPD);杂交子代;杂种优势;PIT标记
基金项目(Foundation): 亚洲开发银行区域合作项目(ADB-RETA6136)。
作者(Author): 佟雪红,董在杰,缪为民,袁新华,王建新,岳春梅,林连升
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