刘世婷,苏境坦,刘骏,黄晶,方东,谢莉萍,王洪钟,张贵友,杜晓东,麻彩萍,张荣庆

• 1:清华大学生命科学学院
• 2:广东海洋大学水产学院

摘要(Abstract)：

通过预测、筛选并合成合浦珠母贝无定形碳酸钙结合蛋白(ACCBP)潜在的功能性肽段,发现位于其C末端含乙酰胆碱结合位点保守区域的多肽具有显著的生物矿化功能;体外碳酸钙结晶速度实验显示该多肽能够延缓碳酸钙的结晶;体外碳酸钙结晶实验显示其能够显著影响方解石晶体的晶貌,使得方解石晶体的边缘呈现出阶梯状台阶现象;体外模拟间液离子环境碳酸钙结晶实验显示其能够抑制文石晶体成核或促进已成核文石晶体生长,使得实验组中的文石晶体颗粒数量显著减少并多呈现球形。结果提示了ACCBP上乙酰胆碱结合位点不仅能够直接参与生物矿化,并且很可能是其参与矿化的重要区域。

关键词(KeyWords)： 生物矿化;无定形碳酸钙结合蛋白(ACCBP);功能性肽段;乙酰胆碱结合位点

Abstract:

Keywords:

基金项目(Foundation): 2008年教育部博士点新教师基金(200800031014)

作者(Author): 刘世婷,苏境坦,刘骏,黄晶,方东,谢莉萍,王洪钟,张贵友,杜晓东,麻彩萍,张荣庆

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