崔雁冰,陈绍红,刘铀,刘艳芬,赵云涛

• 1:广东海洋大学食品科技学院
• 2:广东海洋大学农学院

摘要(Abstract)：

利用长春新碱致神经病理性疼痛小鼠模型,以腹腔注射不同剂量虾青素,通过行为学实验测试机械性缩足反射阈值的变化,并以免疫荧光检测星型胶质细胞和小胶质细胞各自的标记蛋白GFAP和Iba1的蛋白表达变化,以ELISA试剂盒检测炎症因子肿瘤坏死因子α(TNF-α)和单核细胞趋化蛋白1(MCP-1)的表达变化,以此探讨虾青素对长春新碱诱导的神经病理性疼痛的影响及其作用机制。结果表明,虾青素呈剂量依赖性地拮抗长春新碱诱导的神经病理性疼痛(chemotherapy-induced neuropathic pain,CNP)(P<0.01),虾青素能够降低CNP小鼠脊髓胶质细胞GFAP和Iba1的蛋白表达水平以及炎症因子TNF-α和MCP-1的表达水平(P<0.05)。虾青素能显著拮抗长春新碱诱导的神经病理性疼痛。

关键词(KeyWords)： 虾青素;长春新碱;神经病理性疼痛;胶质细胞;炎症因子

Abstract:

Keywords:

基金项目(Foundation): 基金项目:广东海洋大学创新强校工程(GDOU2013050243)

作者(Author): 崔雁冰,陈绍红,刘铀,刘艳芬,赵云涛

参考文献(References)：

• [1]DOUGHERTY P M,CATA J P,BURTON A W,et al.Dysfunction in multiple primary afferent fiber subtypes revealed by quantitative sensory testing in patients with chronic vincristine-induced pain[J].J Pain Symptom Manage,2007,33(2):166-179.
• [2]OKAMOTO K,MARTIN D P,SCHMELZER J D,et al.Pro-and anti-inflammatory cytokine gene expression in rat sciatic nerve chronic constriction injury model of neuropathic pain[J].Exp Neurol,2001,169(2):386-391.
• [3]SCHOLZ J,WOOLF CJ.The neuropathic pain triad:neurons,immune cells and glia[J].Nat Neurosci,2007,10(11):1361-1368.
• [4]LI J J,XIA Y J,LIU T,et al.Protective effects of astaxanthin on Con A-induced autoimmune hepatitis by the JNK/p-JNK pathway-mediated inhibition of autophagy and apoptosis[J].PLo S One,2015,10:e0120440.
• [5]OHGAMI K,SHIRATORI K,KOTAKE S,et al.Effects of astaxanthin on lipopolysaccharide-induced inflammation in vitro and in vivo[J].Invest Ophthalmol Vis Sci,2003,44(6):2694-2701.
• [6]LOBOS P,BRUNA B,CORDOVA A,et al.Astaxanthin protects primary hippocampal neurons against noxious effects of Aβ-oligomers[J].Neural Plast,2016,3456783.
• [7]LEE DH,KIM CS,LEE YJ.Astaxanthin protects against MPTP/MPP+-induced mitochondrial dysfunction and ROS production in vivo and in vitro[J].Food Chem Toxicol,2011,49(1):271-280.
• [8]KUEDO Z,SANGSURIYAWONG A,KLAYPRADIT W,et al.Effects of astaxanthin from Litopenaeus vannamei on carrageenan-induced edema and pain behavior in mice[J].Molecules,2016,21(3):382.
• [9]ZHOU X,ZHANG F,HU X,et al.Inhibition of inflammation by astaxanthin alleviates cognition deficits in diabetic mice[J].Physiol Behav,2015,151:412-420.
• [10]YOOK JS,OKAMOTO M,RAKWAL R,et al.Astaxanthin supplementation enhances adult hippocampal neurogenesis and spatial memory in mice[J].Mol Nutr Food Res,2016,60:589-599.
• [11]SAIKA F,KIGUCHI N,KOBAYASHI Y,et al.Suppressive effect of imipramine on vincristine-induced mechanical allodynia in mice[J].Biol Pharm Bull,2009,32(7):1231-1234.
• [12]胡传银,丁银润.大鼠鞘内虎耳草素抗神经病理性疼痛效果分析[J].现代医药卫生,2012,28(12):62-63.
• [13]SHEN Y,ZHANG Z J,ZHU M D,et al.Exogenous induction of HO-1 alleviates vincristine-induced neuropathic pain by reducing spinal glial activation in mice[J].Neurobiol Dis,2015,79:100-110.
• [14]ANDERSSON D A,GENTRY C,MOSS S,et al.Transient receptor potential A1 is a sensory receptor for multiple products of oxidative stress[J].J Neurosci,2008,28(10):2485-2494.
• [15]DAI Y,WANG S,TOMINAGA M,et al.Sensitization of TRPA1 by PAR2 contributes to the sensation of inflammatory pain[J].J Clin Invest,2007,117(7):1979-1987.