陶甜甜,焦学,甘萍,俞国豪,崔仟,潘明轩,胡亚东,杨光

• 1:江苏海洋大学海洋食品与生物工程学院
• 2:江苏海洋生物资源创新中心

摘要(Abstract)：

【目的】筛选产高活性壳聚糖酶的菌株，探究菌株产壳聚糖酶的酶学性质及高效制备壳寡糖的工艺条件。【方法】从连云港燕尾港海域的底泥中采用透明圈法和酶活性测定分离并筛选高产壳聚糖酶菌株；利用分级沉淀、层析方法纯化壳聚糖酶，并测定其酶学性质；以壳聚糖水解率及壳寡糖得率为指标，采用单因素试验法优化壳聚糖酶高效制备壳寡糖的条件。【结果与结论】获得一株产高活性壳聚糖酶[活性为（245.22±3.18）U/mL]的芽孢杆菌属Bacillus sp. CJB-H6菌株；纯化后的壳聚糖酶（Chitosanase from Bacillus sp. CJB-H6,Bpt CSN）分子质量约为34kDa，比活性高达（9 889.33±47.21）U/mg，最适催化温度为55℃，最适pH为6.0；金属离子Ba~(2+)和Mg~(2+)对Bpt CSN活性有显著促进作用（P<0.05）。在55℃下，Bpt CSN催化60 g/L胶体壳聚糖的最适工艺条件为加酶量150 U/mL、乙酸体积分数2.0%、转速100 r/min、酶解2.5 h。在此工艺条件下制备壳寡糖，壳聚糖水解率和壳寡糖得率分别为94.69%±5.38%和91.06%±4.91%，酶解产物主要为壳二糖、壳三糖和壳四糖。

关键词(KeyWords)： 壳聚糖酶;产酶菌株;芽孢杆菌属;酶学性质;壳寡糖

Abstract:

Keywords:

基金项目(Foundation): 江苏省高等学校基础科学研究面上项目（24KJB180003）;; 江苏省青年科技人才托举工程项目（JSTJ-2024-168）;; 江苏省海洋资源开发技术创新中心海洋科技项目（LWKJ-07）;; 江苏海洋生物资源创新中心海洋创新项目（JOUH23604）

作者(Author): 陶甜甜,焦学,甘萍,俞国豪,崔仟,潘明轩,胡亚东,杨光

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