颜鹏,简纪常,吴灶和

• 1:广东海洋大学水产学院
• 2:广东省水产经济动物病原生物学及流行病学重点实验室
• 3:广东省水产经济动物病害控制重点实验室
• 4:仲恺农业工程学院

摘要(Abstract)：

白细胞介素6(IL-6)是一个多效的细胞因子,在机体免疫应答、急性期反应以及造血调控等过程中发挥着重要作用。以草鱼(Ctenopharyngodon idella)为研究对象,采用RT-PCR和Smart RACE技术克隆获得草鱼IL-6(CiIL-6)cDNA序列,CiIL-6的cDNA全长为1 145 bp,包含一个长为702 bp的开放阅读框,能编码233个氨基酸,预测CiIL-6的蛋白质分子质量为26.74 ku,等电点为8.72。其中5'和3'非编码区(UTR)分别为86 bp和357bp。氨基酸同源性分析显示,草鱼和斑马鱼(Danio rerio)的亲缘关系最近,它们的CiIL-6氨基酸同源性高达76%,而与其他物种的同源性均低于30%。RT-PCR的结果显示,CiIL-6在健康草鱼的胸腺、头肾和脾脏表达最高,而在鳃、胃和心脏中的表达量最低。

关键词(KeyWords)： 草鱼;白细胞介素6;基因克隆;基因表达;RACE

Abstract:

Keywords:

基金项目(Foundation): 973计划“水生动物呼肠孤病毒的致病机理与保护性抗原研究”(2009CB118704)

作者(Author): 颜鹏,简纪常,吴灶和

参考文献(References)：

• [1]Choy E H,Isenberg D A,Garrood T,et al.Therapeutic benefit ofblocking interleukin-6 activity with an anti-interleukin-6 receptormonoclonal antibody in rheumatoid arthritis:a randomized,double-blind,placebo-controlled,dose-escalation trial[J].ArthritisRheum,2002,46(12):43-50.
• [2]Wong G C,Witek-Gianotti J S,Temple P A,et al.Stimulation ofmurine hemotopietic colony formation by human IL-6[J].J Immunol,1988,140(9):3040-3044.
• [3]Wang Jing-yan.Detection of serum TNF-α,IFN-γ,IL-6 and IL-8 inpatients with hepatitis B[J].World Journal of Gastroenterology,1999,5(1):38-40.
• [4]陈耿,华利忠,张书霞.猪圆环病毒Ⅱ和巨噬细胞对体外培养仔猪淋巴细胞IL-6和IL-10及其受体表达的影响[J].江苏农业学报,2010,26(5):993-998.
• [5]陈真真,陈宗波,赵娜,等.肠道病毒71型感染小鼠IL-6,TNF-α和脑细胞凋亡的影响[J].现代生物医学进展,2010,10(18):3419-3422.
• [6]Haegeman G,Content J,Volckaert G,et al.Structural analysis of thesequence coding for an inducible 26 KDa protein in humanfibroblasts[J].Eur J Biochem,1986,159(3):625–632.
• [7]金伯泉.细胞和分子免疫学[M].北京:世界图书出版公司,1995:108-112.
• [8]Mehler M F,Kessler J A.Hematolymphopoietic and inflammatorycytokines in neural development[J].Trends Neurosci,1997,20(8):357–365.
• [9]Varela M,Dios S,Novoa B,et al.Characterization expression andontogeny of interleukin-6 and its receptors in zebrafish(Daniorerio)[J].Developmental and Comparative Immunology,2012,37(1):97-106.
• [10]Takai Y,Wong G G,Clark S C,et al.B cell stimulatory factor-2 isinvolved in the differentiation of cytotoxic Tlymphocytes[J].JImmunol,1988,140(2):508-512.
• [11]Okano A,Suzuki C,Takatsuki F,et al.In vitro expansion of themurine pluripotent hemopoietic stem cell population in response tointerleukin 3 and interleukin 6:application to bone marrowtransplantation[J].Transplantation,1989,48(3):495-498.
• [12]Huggett A,Ford C P,Thorgeirsson SS.Effects of interleukin-6 on thegrowth of normal and transformed rat liver cells in culture[J].GrowthFactors,1989,2(1):83-89.
• [13]Hirano T.Interleukin 6 and its receptor:ten years later[J].Int RevImmunol,1998,16(3-4):249-84.
• [14]Szelényi J.Cytokines and the central nervous system[J].Brain ResBull,2001,54(4):329–38.
• [15]李冰,马颂华,邱一华.白细胞介素-6受体在大鼠小脑顶核中的表达[J].交通医学,2009,23(6):599-601.
• [16]Ahne W.Presence of interleukins(IL-1,IL-3,IL-6)and tumornecrosis factor(TNF alpha)in fish sera[J].Bulletin of the EuropeanAssociation of Fish Pathologists,1993,13(3):106-107.
• [17]Beck G,Habicht G S.Characterization of an IL-6-like molecule froman echinoderm(Asterias forbesi)[J].Cytokine,1996,8(7):507–512.
• [18]Ahne W.Lectin(Con A)induced interleukin(IL-1 alpha,IL2,IL6)production in vitro by leukocytes of rainbow trout(Oncorhynchusmykiss)[J].Bulletin of the European Association of Fish Pathologists,1994,14(1):33-35.
• [19]Bird S,Zou J,Savan R,et al.Characterisation and expressionanalysis of an interleukin 6 homologue in the Japanese pufferfish,Fugu rubripes[J].Dev Comp Immunol,2005,29(9):775–789.
• [20]Castellanaa B,Iliev D B,Sepulcre M P.Molecular characterization ofinterleukin-6 in the gilthead seabream(Sparus aurata)[J].MolecularImmunology,2008,45(12):3363–3370.
• [21]Iliev D B,Castellanaa B,Mackenzie S.Cloning and expressionanalysis of an IL-6 homolog in rainbow trout(Oncorhynchusmykiss)[J].Molecular Immunology,2007,44(7):1803–1807.
• [22]Varela M,Dios S,Novoa B,et al.Charaterisation,expression andontogeny of interleukin-6 and its receptors in zebrafish(Danio rerio)[J].Dev Comp Immunol,2012,37(1):97-106.
• [23]Wang T H,Huang W S,Costa M M,et al.The gamma-chaincytokine/receptor system in fish:more ligands and receptors[J].Fish&Shellfish Immunology,2011,31(5):673-687.
• [24]Huising M O,Stet R J,Savelkoul H F,et al.The molecular evolutionof the interleukin-1 family of cytokines;IL-18 in teleost fish[J].DevComp Immunol,2004,28(5):395–44.
• [25]Nam B H,Byon J Y,Kim Y O,et al.Molecular cloning andcharacterisation of the flounder(Paralichthys olivaceus)interleukin-6gene[J].Fish&Shellfish Immunology,2007,23(1):231-236.