马氏珠母贝肠道及其养殖水体可培养细菌群落结构Community Structure of Culturable Bacterial Communities in the Intestine of Pinctada fucata martensii and Its Aquaculture Water
陈琨,黄湾,廖永山,杨创业,王庆恒
摘要(Abstract):
【目的】了解马氏珠母贝(Pinctada fucata martensii)肠道及其养殖水体可培养细菌的群落组成。【方法】采用2216E平板涂布法研究海区养殖马氏珠母贝肠道与养殖水体的可培养菌群种类及丰度。【结果与结论】马氏珠母贝肠道及其养殖水体的可培养细菌归属于2门(变形菌门和厚壁菌门)3纲7目10科23属56种。属水平上,肠道中以弧菌属(74.7%)和假交替单胞菌属(18.7%)为主;养殖水体中α-变形菌纲的FJ943236_g属(40.7%)丰度最大,弧菌属(16.7%)相对肠道丰度较低。样品共有菌属为弧菌属、假交替单胞菌属、发光杆菌属和芽孢杆菌属;肠道特有菌属为希瓦氏菌属和盐单胞菌属;养殖水体特有菌属主要为FJ943236_g、鲁杰氏菌属和Nautella。在种水平上,7个种为二者共有;马氏珠母贝肠道和养殖水体特异性菌种分别为18个和31个。虽然门水平上马氏珠母贝肠道中可培养细菌群落与其养殖水体中的细菌群落大致相似,但在属、种水平上二者差异明显。
关键词(KeyWords): 马氏珠母贝;肠道菌群;养殖水体菌群;16S rRNA基因
基金项目(Foundation): 广东省自然科学基金(2020A151501691);; 广东省现代农业产业技术体系贝藻类产业创新团队(2019KJ146)
作者(Author): 陈琨,黄湾,廖永山,杨创业,王庆恒
参考文献(References):
- [1]INGERSLEV H C,J?RGENSEN L V G,STRUBE M L,et al.The development of the gut microbiota in rainbow trout(Oncorhynchus mykiss) is affected by first feeding and diet type[J].Aquaculture,2014,424/425:24-34.
- [2]高权新,吴天星,王进波.肠道微生物与寄主的共生关系研究进展[J].动物营养学报,2010,22(3):519-526.
- [3]MOORE A,MUNCK C,SOMMER M O A,et al.Functional metagenomic investigations of the human intestinal microbiota[J].Frontiers in Microbiology,2011,2:188.
- [4]MORAN D,TURNER S J,CLEMENTS K D.Ontogenetic development of the gastrointestinal microbiota in the marine herbivorous fish Kyphosus sydneyanus[J].Microbial Ecology,2005,49(4):590-597.
- [5]WU S G,WANG G T,ANGERT E R,et al.Composition,diversity,and origin of the bacterial community in grass carp intestine[J].PLo S One,2012,7(2):e30440.
- [6]孙敬锋,贾艳丽.鱼类肠道菌群与免疫调节研究进展[J].水产科技情报,2016,43(2):96-100.
- [7]郑艺,张家超,郭壮,等.基于高通量测序技术分析肠道菌群及其影响因素的研究进展[J].中国食品学报,2014,14(11):157-164.
- [8]李志勇,何丽明,吴杰,等.基于PCR-DGGE基因指纹的对虾体内优势细菌组成分析[J].微生物学通报,2005,32(3):82-86.
- [9]GILL S R,POP M,DEBOY R T,et al.Metagenomic analysis of the human distal gut microbiome[J].Science,2006,312(5778):1355-1359.
- [10]RAWLS J F,SAMUEL B S,GORDON J I.Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota[J].Proceedings of the National Academy of Sciences of the United States of America,2004,101(13):4596-4601.
- [11]李英英,陈曦,宋铁英.不同生长速度的大黄鱼肠道菌群结构的差异[J].大连海洋大学学报,2017,32(5):509-513.
- [12]MERRIFIELD D L,BURNARD D,BRADLEY G,et al.Microbial community diversity associated with the intestinal mucosa of farmed rainbow trout(Oncoryhnchus mykiss Walbaum)[J].Aquaculture Research,2009,40(9):1064-1072.
- [13]THOMPSON-CHAGOYáN O C,MALDONADO J,GIL A.Colonization and impact of disease and other factors on intestinal microbiota[J].Digestive Diseases and Sciences,2007,52(9):2069-2077.
- [14]吴金凤,熊金波,王欣,等.肠道菌群对凡纳滨对虾健康的指示作用[J].应用生态学报,2016,27(2):611-621.
- [15]CARDONA E,GUEGUEN Y,MAGRéK,et al.Bacterial community characterization of water and intestine of the shrimp Litopenaeus stylirostris in a biofloc system[J].BMC Microbiology,2016,16(1):1-9.
- [16]王贤丰,赵艳飞,宋志飞,等.应用高通量测序技术分析拟穴青蟹肠道及其养殖环境菌群结构[J].中国水产科学,2017,24(6):1245-1253.
- [17]POWELL S M,CHAPMAN C C,BERMUDES M,et al.Dynamics of seawater bacterial communities in a shellfish hatchery[J].Microbial Ecology,2013,66(2):245-256.
- [18]赵晓美,桑雪,张公亮,等.大长山岛虾夷扇贝养殖海区及其体内细菌数量周年变化[J].大连工业大学学报,2015,34(2):84-87.
- [19]吴欢欢,王伟继,吕丁,等.应用高通量测序技术分析大菱鲆幼鱼肠道及其养殖环境的微生物群落结构[J].渔业科学进展,2019,40(4):84-94.
- [20]李靖,廖梅杰,李彬,等.养殖刺参早期发育阶段体内可培养细菌的菌群特征及其与环境菌群相关性分析[J].渔业科学进展,2019,40(2):122-131.
- [21]张素萍.中国海洋贝类图鉴[M].海洋出版社,2008.
- [22]LIAO Y S,CAI C X,YANG C Y,et al.Effect of protein sources in formulated diets on the growth,immune response,and intestinal microflora of pearl oyster Pinctada fucata martensii[J].Aquaculture Reports,2020,16:100253.
- [23]GODOY F.Characterization of culturable bacteria in larval cultures of the Chilean scallop Argopecten purpuratus[J].Ciencias Marinas,2011,37(3):339-348.
- [24]GONZALEZ A,STOMBAUGH J,LOZUPONE C,et al.The mind-body-microbial continuum[J].Dialogues in Clinical Neuroscience,2011,13(1):55-62.
- [25]WU S G,GAO T H,ZHENG Y Z,et al.Microbial diversity of intestinal contents and mucus in yellow catfish(Pelteobagrus fulvidraco)[J].Aquaculture,2010,303(1/2/3/4):1-7.
- [26]孙雪莹.虾夷扇贝幼体及育苗池水体细菌群落动态及潜在益生菌筛选[D].大连:大连海洋大学,2016.
- [27]SANDAA R A,MAGNESEN T,TORKILDSEN L,et al.Characterisation of the bacterial community associated with early stages of Great Scallop (Pecten maximus),using denaturing gradient gel electrophoresis(DGGE)[J].Systematic and Applied Microbiology,2003,26(2):302-311.
- [28]丁君,窦妍,徐高蓉,等.基于454焦磷酸测序分析虾夷扇贝外套膜菌群多样性[J].应用生态学报,2014,25(11):3344-3348.
- [29]TRABAL FERNáNDEZ N,MAZóN-SUáSTEGUI JM,VáZQUEZ-JUáREZ R,et al.Changes in the composition and diversity of the bacterial microbiota associated with oysters (Crassostrea corteziensis,Crassostrea gigas and Crassostrea sikamea) during commercial production[J].FEMS Microbiology Ecology,2014,88(1):69-83.
- [30]李明,刘继晨,孙雪莹,等.育苗阶段虾夷扇贝幼体中可培养细菌的多样性[J].大连海洋大学学报,2016,31(4):374-379.
- [31]SCHULZE A D,ALABI A O,TATTERSALL-SHELDRAKEA R,et al.Bacterial diversity in a marine hatchery:Balance between pathogenic and potentially probiotic bacterial strains[J].Aquaculture,2006,256(1):50-73.
- [32]SCHULZE A D,ALABI A O,TATTERSALL-SHELDRAKEA R,et al.Bacterial diversity in a marine hatchery:Balance between pathogenic and potentially probiotic bacterial strains[J].Aquaculture,2006,256(1):50-73.
- [33]NAJIAH M,NADIRAH M,LEE K L,et al.Bacteria flora and heavy metals in cultivated oysters Crassostrea iredalei of Setiu Wetland,East Coast Peninsular Malaysia[J].Veterinary Research Communications,2008,32(5):377-381.
- [34]肖建青,朱泓溢,刘祝祥,等.硇洲岛潮汐带牡蛎相关可培养细菌多样性[J].微生物学通报,2013,40(6):939-950.
- [35]LIU J C,SUN X Y,LI M,et al.Vibrio infections associated with yesso scallop(Patinopecten yessoensis)larval culture[J].Journal of Shellfish Research,2015,34(2):213-216.
- [36]PUJALTE M J,ORTIGOSA M,MACIAN M C,et al.Aerobic and facultative anaerobic heterotrophic bacteria associated to Mediterranean oysters and seawater[J].International Microbiology,1999,2(4):259-266.
- [37]孙盛明,苏艳莉,张武肖,等.饲料中添加枯草芽孢杆菌对团头鲂幼鱼生长性能、肝脏抗氧化指标、肠道菌群结构和抗病力的影响[J].动物营养学报,2016,28(2):507-514.
- [38]杨明容,李达,周美玉.饲用抗生素替代物在水产养殖中的应用研究进展[J].安徽农学通报,2020,26(8):62-66.
- [39]ARANDA C P,VALENZUELA C,BARRIENTOS J,et al.Bacteriostatic anti-Vibrio parahaemolyticus activity of Pseudoalteromonas sp.strains DIT09,DIT44 and DIT46 isolated from Southern Chilean intertidal Perumytilus purpuratus[J].World Journal of Microbiology and Biotechnology,2012,28(6):2365-2374.
- [40]KESARCODI-WATSON A,MINER P,NICOLAS J,et al.Protective effect of four potential probiotics against pathogen-challenge of the larvae of three bivalves:Pacific oyster (Crassostrea gigas),flat oyster (Ostrea edulis) and scallop (Pecten maximus)[J].Aquaculture,2012,344:29-34.
- [41]TORKILDSEN L,LAMBERT C,NYLUND A,et al.Bacteria associated with early life stages of the great scallop,Pecten maximus:impact on larval survival[J].Aquaculture International,2005,13(6):575-592.
- [42]SUN K,ZHANG W W,HOU J H,et al.Immunoprotective analysis of Vhh P2,a Vibrio harveyi vaccine candidate[J].Vaccine,2009,27(21):2733-2740.
- [43]GOLDSCHMIDT-CLERMONT E,WAHLI T,FREY J,et al.Identification of bacteria from the normal flora of perch,Perca fluviatilis L.,and evaluation of their inhibitory potential towards Aeromonas species[J].Journal of Fish Diseases,2008,31(5):353-359.
- [44]FJELLHEIM A J,PLAYFOOT K J,SKJERMO J,et al.Vibrionaceae dominates the microflora antagonistic towards Listonella anguillarum in the intestine of cultured Atlantic cod (Gadus morhua L.) larvae[J].Aquaculture,2007,269(1/2/3/4):106.