王维政,曾泽乾,黄建盛,郭志雄,李洪娟,陈刚

• 1:广东海洋大学水产学院
• 2:南方海洋科学与工程广东省实验室(湛江)

摘要(Abstract)：

【目的】研究低氧胁迫对军曹鱼(Rachycentron canadum)幼鱼抗氧化、免疫能力和能量代谢的影响。【方法】幼鱼于(2.98±0.40) mg/L的低溶氧条件下养殖1周,分别测定其肝脏和肌肉组织的抗氧化、免疫相关酶活力以及能量供应物质。【结果】低氧胁迫过程中,幼鱼肝脏组织超氧化物歧化酶(SOD)活力显著升高(P <0.05)后逐渐下降(P <0.05),肌肉组织SOD活力呈波动上升趋势(P <0.05),肝脏和肌肉组织谷胱甘肽过氧化物酶(GPX)活力和丙二醛(MDA)含量均显著升高(P<0.05)后,呈逐渐下降趋势,肝脏组织过氧化氢酶(CAT)先降(P<0.05)后升至正常水平,肌肉组织CAT活力先升(P <0.05)后呈下降趋势;肝脏组织碱性磷酸酶(AKP)活力先降后升高(P <0.05),随后恢复至与对照组水平(P> 0.05),肝脏酸性磷酸酶(ACP)活力先显著上升(P <0.05),随后恢复至正常水平(P> 0.05);肌肉组织和肝脏组织乳酸脱氢酶(LDH)活力变化趋势一致,均为先显著升高(P<0.05),之后呈降低趋势;肝糖原在低氧胁迫后呈先下降(P <0.05),后恢复至对照组水平(P> 0.05),肌糖原各时间点含量无显著性差异(P> 0.05)。【结论】军曹鱼幼鱼在低氧胁迫后发生氧化损伤,刺激自身免疫系统,通过调整相关酶活力及能量代谢的方式提高其适应低氧的能力。

关键词(KeyWords)： 军曹鱼;低氧胁迫;抗氧化;免疫;能量代谢

Abstract:

Keywords:

基金项目(Foundation): 现代农业产业技术体系专项资金资助(CARS-47);; 广东海洋大学大学生创新创业训练计划项目(CXXL2018131);; 南方海洋科学与工程广东省实验室(湛江)(ZJW-2019-06);; 2018年广东海洋大学起航计划项目(QHJHZR201818)

作者(Author): 王维政,曾泽乾,黄建盛,郭志雄,李洪娟,陈刚

参考文献(References)：

• [1]穆景利,靳非,赵化德,等.水体低氧的早期暴露对青鳉(Oryzias latipes)后期的生长、性别比和繁殖能力的影响[J].生态毒理学报,2017,12(2):137-146.
• [2]徐贺,陈秀梅,王桂芹,等.低氧胁迫在水产养殖中的研究进展[J].饲料工业,2016,37(2):33-37.
• [3]狄治朝,周涛,许强华.低氧胁迫与常氧条件下斑马鱼鳃中热休克蛋白基因家族的表达差异比较[J].大连海洋大学学报,2018,33(6):690-695.
• [4]DU S N,MAHALINGAM S,BOROWIEC B G,et al.Mitochondrial physiology and reactive oxygen species production are altered by hypoxia acclimation in killifish(Fundulus heteroclitus)[J].Journal of Experimental Biology,2016,219(8):1130-1138.
• [5]WANG Q F,SHEN W L,HOU C C,et al.Physiological responses and changes in gene expression in the large yellow croaker Larimichthys crocea following exposure to hypoxia[J].Chemosphere,2017,169:418-427.
• [6]ZHANG G S,ZHAO C,WANG Q T,et al.Identification of HIF-1 signaling pathway in Pelteobagrus vachelli using RNA-Seq:effects of acute hypoxia and reoxygenation on oxygen sensors,respiratory metabolism,and hematology indices[J].Journal of Comparative Physiology B,2017,187(7):1-13.
• [7]王中铎,史沛鑫,苏惠娜,等.军曹鱼群体遗传结构的AFLP分析[J].广东海洋大学学报,2011,31(1):12-17.
• [8]陈强,刘泓宇,谭北平,等.饲料胆固醇对军曹鱼幼鱼生长、血液生化指标及脂代谢的影响[J].广东海洋大学学报,2016,36(1):35-43.
• [9]郭志雄,曾泽乾,黄建盛,等.急性低氧胁迫对大规格军曹鱼幼鱼肝脏氧化应激、能量利用及糖代谢的影响[J].广东海洋大学学报,2020,40(3):134-140.
• [10]黄建盛,陆枝,陈刚,等.急性低氧胁迫对军曹鱼大规格幼鱼血液生化指标的影响[J].海洋学报,2019,41(6):76-84.
• [11]李洪娟,陈刚,郭志雄,等.军曹鱼(Rachycentron canadum)幼鱼对环境低氧胁迫氧化应激与能量利用指标的响应[J].海洋学报,2020,42(4):12-19.
• [12]LUSHCHAK V I,BAGNYUKOVA T V.Hypoxia induces oxidative stress in tissues of a goby,the rotan Perccottus glenii[J].Comparative Biochemistry&Physiology Part B,2007,148(4):390-397.
• [13]ZHANG G S,MAO J Q,LIANG F F,et al.Modulated expression and enzymatic activities of Darkbarbel catfish,Pelteobagrus vachelli for oxidative stress induced by acute hypoxia and reoxygenation[J].Chemosphere,2016,151:271-279.
• [14]DASGUPTA S,GIULIO R T D,DROLLETTE B D,et al.Hypoxia depresses CYP1A induction and enhances DNA damage,but has minimal effects on antioxidant responses in sheepshead minnow(Cyprinodon variegatus)larvae exposed to dispersed crude oil[J].Aquatic Toxicology,2016,177:250-260.
• [15]CAO L,HUANG W,SHAN X J,et al.Tissue-specific accumulation of cadmium and its effects on antioxidative responses in Japanese flounder juveniles[J].Environmental Toxicology&Pharmacology,2012,33(1):16-25.
• [16]FENG J F,GUO Y,GAO Y F,et al.Effects of hypoxia on the physiology of zebrafish(Danio rerio):Initial responses,acclimation and recovery[J].Bulletin of Environmental Contamination&Toxicology,2016,96(1):43-48.
• [17]贾秀琪,张宏叶,王丽等.低氧胁迫对河川沙塘鳢抗氧化酶及ATP酶活性的影响[J].海洋渔业,2017,39(3):306-313.
• [18]YANG S,YAN T,WU H,et al.Acute hypoxic stress:effect on blood parameters,antioxidant enzymes,and expression of HIF-1alpha and GLUT-1 genes in largemouth bass(Micropterus salmoides)[J].Fish&Shellfish Immunology,2017,67:449-458.
• [19]LEVEELAHTI L,RYTK?NEN K T,RENSHAW G MC,et al.Revisiting redox-active antioxidant defenses in response to hypoxic challenge in both hypoxia-tolerant and hypoxia-sensitive fish species[J].Fish Physiology&Biochemistry,2014,40(1):183-191.
• [20]区又君,陈世喜,王鹏飞,等.低氧环境下卵形鲳鲹的氧化应激响应与生理代谢相关指标的研究[J].南方水产科学,2017,13(3):120-124.
• [21]李洁,唐夏,张灵燕等.溶解氧水平对褐牙鲆幼鱼能量代谢和氧化应激的影响研究[J].广西科学院学报,2015,31(1):22-27.
• [22]PILLET M,DUPONTPRINET A,CHABOT D,et al.Effects of exposure to hypoxia on metabolic pathways in northern shrimp(Pandalus borealis)and Greenland halibut(Reinhardtius hippoglossoides)[J].Journal of Experimental Marine Biology&Ecology,2016,483:88-96.
• [23]GUO J S,WU P H,CAO J L,et al.The PFOS disturbed immunomodulatory functions via nuclear factor-κBsignaling in liver of zebrafish(Danio rerio)[J].Fish&shellfish immunology,2019,91:87-98.
• [24]CHEN S J,XIE S W,CHEN M,et al.Hypoxia-induced changes in survival,immune response and antioxidant status of the Pacific white shrimp(Litopenaeus vannamei)fed with graded levels of dietary myo-inositol[J].Aquaculture nutrition,2019,25(2):518-528.
• [25]MA J G,LI Y Y,WU M L,et al.Serum immune responses in common carp(Cyprinus carpio L.)to paraquat exposure:The traditional parameters and circulating micro RNAs[J].Fish&Shellfish Immunology,2018,76:133-142.
• [26]KHAIRNAR S O,TIAN X,DONG S,et al.Effects of the amplitude and frequency of salinity fluctuations on antioxidant responses in juvenile tongue sole,Cynoglossus semilaevis[J].Spanish Journal of Agricultural Research,2016,14(2):e0503.
• [27]WANG Y J,LI W M,LI L S,et al.Effects of salinity on the physiological responses of the large yellow croaker Pseudosciaena crocea under indoor culture conditions[J].Aquaculture Research,2016,47(11):3410-3420.
• [28]常志成,温海深,张美昭,等.溶解氧水平对花鲈幼鱼氧化应激与能量利用的影响及生理机制[J].中国海洋大学学报(自然科学版),2018,48(7):20-28.
• [29]SPEERS R B,SANDBLOM E G.Effects of environmental hypoxia on cardiac energy metabolism and performance in tilapia[J].Am J Physiol Regul Integr Comp Physiol,2010,298(2):104-119.
• [30]LI M X,WANG X D,QI C L,et al.Metabolic response of Nile tilapia(Oreochromis niloticus)to acute and chronic hypoxia stress[J].Aquaculture,2018,495:187-195.
• [31]YANG S,WU H,HE K,et al.Response of AMP-activated protein kinase and lactate metabolism of largemouth bass(Micropterus salmoides)under acute hypoxic stress[J].Science of the Total Environment,2019,666:1071-1079.
• [32]WANG Q F,SHEN W L,LIU C,et al.Effects of multi-environmental factors on physiological and biochemical responses of large yellow croaker,Larimichthys crocea[J].Chemosphere,2017,184:907-915.