闫秀英,郭定利,郑树城,王雅,谢吉国,简纪常,吴灶和

• 1:广东海洋大学水产学院
• 2:广东省水产经济动物病原生物学及流行病学重点实验室暨水产经济动物病害控制广东普通高校重点实验室
• 3:上海海洋大学水产与生命学院
• 4:北京市大兴区水产技术推广站

摘要(Abstract)：

克隆得一株新分离病毒草鱼呼肠孤病毒(GCRV)096的长为1 981 bp的vp4基因,分析该基因的生物信息及其编码蛋白的特性。生物信息学分析表明,同一基因型GCRV分离株vp4基因间的差异不大,但不同基因型GCRV分离株vp4基因间却存在很大的差异。GCRV 096 VP4蛋白含有多个功能位点、2个跨膜结构域及多个潜在的抗原决定簇。构建p EGFP-N3-VP4真核表达质粒,并转染至草鱼肾(Ctenopharyngodon idellus kidney,CIK)细胞中,经荧光显微镜观察和Western blot鉴定,表明GCRV 096 VP4融合蛋白在CIK细胞中得以成功表达。

关键词(KeyWords)： 草鱼呼肠孤病毒;vp4基因;生物信息学;真核表达

Abstract:

Keywords:

基金项目(Foundation): 国家973计划项目(2009CB118704)

作者(Author): 闫秀英,郭定利,郑树城,王雅,谢吉国,简纪常,吴灶和

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