白俊杰,刘碧莲,劳海华,叶星,简清

• 1:中国水产科学研究院珠江水产研究所 中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室
• 2:中国水产科学研究院珠江水产研究所
• 3:中国水产科学研究院热带亚热带鱼类选育与养殖重点开放实验室
• 4:上海水产大学生命科学与技术学院

摘要(Abstract)：

以大口黑鲈为材料,提取肝脏总RNA,经RT-PCR扩增出hepcidin cDNA的开放阅读框(ORF)及3′端非编码区序列,应用5′RACE方法得到大口黑鲈hepcidin cDNA5′末端。将所获得的两个片段分别克隆到T载体后进行测序,并拼接成大口黑鲈hepcidin全长cDNA。序列分析表明:大口黑鲈hepcidin全长cDNA为564bp,含有一个258bp的ORF,编码86个氨基酸残基,由信号肽(24个残基)、前肽(42个残基)和成熟肽(20个残基)3部分组成hepcidin前体。在前肽部分具有前肽转化酶典型的RX(K/R)R基元,成熟肽部分含有8个保守的半胱氨酸残基,可形成四个链内二硫桥,使β-折叠结构保持稳定。大口黑鲈Hepcidin与其他鱼类的同源性在29.7%~90.5%间,尤其是信号肽区域,与鳜、尼罗罗非鱼、真鲷、花鲈、黑鯛、金眼狼鲈仅有2~3个氨基酸的差别。

关键词(KeyWords)： 大口黑鲈;抗菌肽;hepcidin;cDNA克隆;序列分析

Abstract:

Keywords:

基金项目(Foundation): 国家科技支撑项目(NO.2005DKA21103);; 国家科技基础条件平台工作项目(2005DKA21103)

作者(Author): 白俊杰,刘碧莲,劳海华,叶星,简清

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