黄郁葱,鲁义善,简纪常,吴灶和

• 1:中国科学院南海海洋研究所
• 2:中国科学院大学
• 3:广东海洋大学水产学院
• 4:广东省水产经济动物病原生物学及流行病学重点实验室

摘要(Abstract)：

白细胞介素-1受体相关激酶4(interleukin-1 receptor-associated kinase 4,IRAK-4)是一种参与机体先天性免疫和适应性免疫反应过程中的关键分子。采用RT-PCR和c DNA末端快速扩增(RACE-PCR)的方法从红笛鲷(Lutjanus sanguineus)头肾中克隆IRAK-4基因的c DNA全序列(登录号:KF279357)。该序列全长2 015 bp,包含5′非编码区(5′UTR)205 bp,3′非编码区(3′UTR)421 bp,开放阅读框(ORF)1 389 bp,编码462个氨基酸。根据推导的氨基酸序列预测其蛋白分子质量为52.0 ku,理论等电点为5.19。氨基酸序列比对结果显示,红笛鲷IRKA-4基因氨基酸序列与其他物种的同源性为54.2%~85.7%。系统进化分析结果显示,红笛鲷与斜带石斑鱼(Epinephelus coioides)聚为一支,两者有较近的亲缘关系。用荧光定量PCR分析红笛鲷基因的组织表达差异,红笛鲷IRKA-4基因在各组织中均有不同程度的表达,其中在皮肤、肝脏和胃中表达量最高,其次是胸腺、鳃、心脏、肠、肌肉和脾脏,在头肾、后肾和脑的表达量最低。

关键词(KeyWords)： 红笛鲷;IRAK-4;基因克隆;组织表达

Abstract:

Keywords:

基金项目(Foundation): 十二五国家科技支撑计划(2012BAD17B03);; 广东省海洋经济创新发展区域示范专项(GD2012-B01-004);; 国家自然基金(41240041)

作者(Author): 黄郁葱,鲁义善,简纪常,吴灶和

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