李信申,何红,胡汉桥,陈国杰,袁琛

• 1:广东海洋大学农学院
• 2:湛江出入境检验检疫局

摘要(Abstract)：

以大豆病基因类似物(RGA)基因为对照,用RR大豆中的外源CaMV35S启动子、CP4-EPSPS引物,应用多重PCR方法,从RR大豆中扩增出预期大小的DNA片段。结果表明:将扩增产物回收后测序,经同源性分析扩增产物为CaMV35S启动子和CP4-EPSPS的一部分序列;多重PCR检测的灵敏度为0.08%;PCR检测过程中产生假阳性的原因是污染和非特异性扩增。

关键词(KeyWords)： 抗草甘膦;RR大豆;多重PCR

Abstract:

Keywords:

基金项目(Foundation): 广东省湛江出入境检验检疫局课题资助

作者(Author): 李信申,何红,胡汉桥,陈国杰,袁琛

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