罗氏沼虾肝胰腺几丁质酶的亲和—消化纯化方法初探
李友宾
摘要(Abstract)：
关键词(KeyWords)： 罗氏沼虾;几丁质酶;亲和-消化
基金项目(Foundation):
作者(Author): 李友宾
参考文献(References)：
- 1 Arnould C,Jeuniaux C.L es enzymes hydrolytiques du systeme digestif chez les Crustaces Pagurides[J].Cash Biol,1982 ,2 3:89- 10 3
- 2 Brigitte Funke,Klaus- Dieter Spindler. Characterization of chitinase from the brine shrimp Artemia [J]. Comp BiochemPhysiol,1989,94B:6 91- 6 95
- 3 L ynn K.R. Chitinases and chitobioases from the American lobster (H omarusamericanus) [J]. Biochem. Physiol. 1990 ,96 B:76 1- 76 6
- 4 Michiko Kono,Takashi Matshi,Chiaki Shimizu.Purifications and some properties of chitinase from the liver of a prawn,Penaeus japonicus[J].Agric Bio Chem,1990 ,5 4(8) 2 145 - 2 147
- 5 谢雅明 .可溶性甲壳质的制造和用途 [J].化学世界 ,1983,4:118- 12 1
- 6 Molano J,Duran A,Enrico C. A rapid and assay for chitinase using tritiated chitin [J].Anal Biochem,1977,83:6 48
- 7 Reissing JL ,Strominger JL ,L eloir L F. A modified colormetric method for the estimation of N- acetylamino sugar[J]. JBioChem,195 5 ,2 17:95 9
- 8 Bradford M M.A rapid and sensitive method for the quantitation of microgram qualities of protein utilizing the principle ofprotein- dye binding[J].Anal Biochem. 1976 ,72 :2 48- 2 5 4
- 9 Roberts R L ,Cabib E. Serratia marcescens chitinase:one- step purification and use for the determination of chitin[J]. AnalBiochem,1982 ,12 7:40 2 - 412
- 10 Weber K,Osborn M.The reliability ofmolecularweightdeterminations by dodecyl sulfate- polyacrylamind gel electrophoresis[J]. Biol Chem,196 9,2 2 4(16 ) :44 0 6 - 44 12